Stimulation of Cdx1 by oncogenic β-catenin/Tcf4 in colon cancer cells; opposite effect of the CDX2 homeoprotein

被引：26

作者：

Domon-Dell, C ^{[1
]}

Freund, JN ^{[1
]}

机构：

[1] INSERM, U381, F-67200 Strasbourg, France

来源：

FEBS LETTERS | 2002年 / 518卷 / 1-3期

关键词：

homeobox gene; beta-catenin; colon cancer; human;

D O I：

10.1016/S0014-5793(02)02650-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The homeobox gene Cdx1 is a regulator of intestinal epithelial cell proliferation and differentiation. Using a transfection approach, we showed here that the oncogenic activation of the beta-catenin pathway stimulates the endogenous expression of the Cdx1 mRNA as well as the activity of the Cdx1 promoter in cancer cells of the human colon. Reciprocally, the paralogue homeobox gene Cdx2 exerts an inhibitory effect on the basal and on the beta-catenin-stimulated activity of the Cdx1 promoter. The inhibitory effect of CDX2 requires the intact homeodomain. It is not dependent on canonical CDX binding sites in the Cdx1 promoter nor on the cis-elements specifically targeted by the beta-catenin/Tcf complex. We conclude that the oncogenically activated beta-catenin and CDX2 have opposite and independent effects on the Cdx1 homeobox gene. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：83 / 87

页数：5

共 30 条

[11] cis-acting elements and transcription factors involved in the intestinal specific expression of the rat calbindin-D9k gene - Binding of the intestine-specific transcription factor Cdx-2 to the TATA box [J].