Multiplexed Sandwich Immunoassays Using Electrochemiluminescence Imaging Resolved at the Single Bead Level

被引：219

作者：

Deiss, Frederique ^{[1
]}

LaFratta, Christopher N. ^{[2
]}

Symer, Matthew ^{[2
]}

Blicharz, Timothy M. ^{[2
]}

Sojic, Neso ^{[1
]}

Walt, David R. ^{[2
]}

机构：

[1] Univ Bordeaux, Inst Mol Sci, ENSCPB, F-33607 Pessac, France

[2] Tufts Univ, Dept Chem, Medford, MA 02155 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 17期

基金：

美国国家科学基金会;

关键词：

ELECTROGENERATED CHEMILUMINESCENCE; AMPLIFICATION; ARRAYS; SYSTEM;

D O I：

10.1021/ja901876z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new class of bead-based microarray that uses electrogenerated chemiluminescence (ECL) as a readout mechanism to detect multiple antigens simultaneously is presented. This platform demonstrates the possibility of performing highly multiplexed assays using ECL because all the individual sensing beads in the array are simultaneously imaged and individually resolved by ECL. Duplex and triplex assay results are demonstrated as well as a cross reactivity study.

引用

页码：6088 / +

页数：3

共 14 条

[1] Fiber-Optic Microsphere-Based Antibody Array for the Analysis of Inflammatory Cytokines in Saliva [J].

Blicharz, Timothy M. ;

Siqueira, Walter L. ;

Helmerhorst, Eva J. ;

Oppenheim, Frank G. ;

Wexler, Philip J. ;

Little, Frederic F. ;

Walt, David R. .

ANALYTICAL CHEMISTRY, 2009, 81 (06) :2106-2114

[2] A NEW NONISOTOPIC DETECTION SYSTEM FOR IMMUNOASSAYS [J].

DEAVER, DR .

NATURE, 1995, 377 (6551) :758-760

[3] Observing single nanoparticle collisions by electrogenerated chemiluminescence amplification [J].

Fan, Fu-Ren F. ;

Bard, Allen J. .

NANO LETTERS, 2008, 8 (06) :1746-1749

[4] Electrochemiluminescent arrays for cytochrome P450-activated genotoxicity screening.: DNA damage from benzo[a]pyrene metabolites [J].

Hvastkovs, Eli G. ;

So, Minjeong ;

Krishnan, Sadagopan ;

Bajrami, Besnik ;

Tarun, Maricar ;

Jansson, Ingela ;

Schenkman, John B. ;

Rusling, James F. .

ANALYTICAL CHEMISTRY, 2007, 79 (05) :1897-1906

[5] Very high density sensing arrays [J].

LaFratta, Christopher N. ;

Walt, David R. .

CHEMICAL REVIEWS, 2008, 108 (02) :614-637

[6]

*MES SCAL, MES SCAL DIAGN

[7] Electrogenerated chemiluminescence.: 77.: DNA hybridization detection at high amplification with [Ru(bpy)3]2+-containing microspheres [J].

Miao, WJ ;

Bard, AJ .

ANALYTICAL CHEMISTRY, 2004, 76 (18) :5379-5386

[8] Electrogenerated chemiluminescence 69:: The tris(2,2′-bipyridine)ruthenium(II), (Ru(bpy)32+)/tri-n-propylamine (TPrA) system revisited -: A new route involving TPrA•+ cation radicals [J].

Miao, WJ ;

Choi, JP ;

Bard, AJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (48) :14478-14485

[9] Electrogenerated chemiluminescence and its biorelated applications [J].

Miao, Wujian .

CHEMICAL REVIEWS, 2008, 108 (07) :2506-2553

[10] Bioactive protein nanoarrays on nickel oxide surfaces formed by dip-pen nanolithography [J].

Nam, JM ;

Han, SW ;

Lee, KB ;

Liu, XG ;

Ratner, MA ;

Mirkin, CA .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (10) :1246-1249

← 1 2 →