Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P2

被引：229

作者：

Hammond, Gerald R. V. ^{[1
]}

Schiavo, Giampietro ^{[2
]}

Irvine, Robin F. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Pharmacol, Cambridge CB2 1PD, England

[2] Lincolns Inn Fields Labs, Canc Res UK London Res Inst, Mol Neuropathobiol Lab, London WC2A 3PX, England

来源：

BIOCHEMICAL JOURNAL | 2009年 / 422卷

基金：

英国惠康基金;

关键词：

Golgi; immunofluorescence; phosphoinositides; plasma membrane; PtdIns(4,5)P-2; PtdIns4P; PLECKSTRIN HOMOLOGY DOMAIN; PHOSPHATIDYLINOSITOL 4-KINASE ISOFORMS; INOSITOL-POLYPHOSPHATE; 5-PHOSPHATASE; PLASMA-MEMBRANE; ENDOPLASMIC-RETICULUM; BINDING-PROTEIN; HIGH-AFFINITY; FIXED CELLS; GOLGI; LOCALIZATION;

D O I：

10.1042/BJ20090428

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PtdIns4P is the major precursor for the synthesis of the multifunctional plasma membrane lipid, PtdIns(4,5)P-2. Yet PtdIns4P also functions as a regulatory lipid in its own right, particularly at the Golgi apparatus. In the present study we define specific conditions that enable preservation of several organellar membranes for the immunocytochemical detection of PtdIns4P. We report distinct pools of this lipid in both Golgi and plasma membranes, which are synthesized by different PI4K (phosphatidylinositol 4-kinase) activities, and also the presence of PtdIns4P in cytoplasmic vesicles, which are not readily identifiable as PI4K containing trafficking intermediates. In addition, we present evidence that the majority of PtdIns4P resides in the plasma membrane, where it is metabolically distinct from the steady-state plasma membrane pool of PtdIns(4,5)P-2.

引用

页码：23 / 35

页数：13

共 56 条

[21] GAP43, MARCKS, and CAP23 modulate PI(4,5)P2 at plasmalemmal rafts, and regulate cell cortex actin dynamics through a common mechanism [J].

Laux, T ;

Fukami, K ;

Thelen, M ;

Golub, T ;

Frey, D ;

Caroni, P .

JOURNAL OF CELL BIOLOGY, 2000, 149 (07) :1455-1471

[22] SPECIFIC AND HIGH-AFFINITY BINDING OF INOSITOL PHOSPHATES TO AN ISOLATED PLECKSTRIN HOMOLOGY DOMAIN [J].

LEMMON, MA ;

FERGUSON, KM ;

OBRIEN, R ;

SIGLER, PB ;

SCHLESSINGER, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (23) :10472-10476

[23] Targeting of Golgi-specific pleckstrin homology domains involves both PtdIns 4-kinase-dependent and -independent components [J].

Levine, TP ;

Munro, S .

CURRENT BIOLOGY, 2002, 12 (09) :695-704

[24] Regulation of presynaptic phosphatidylinositol 4,5-biphosphate by neuronal activity [J].

Micheva, KD ;

Holz, RW ;

Smith, SJ .

JOURNAL OF CELL BIOLOGY, 2001, 154 (02) :355-368

[25] Phosphatidylinositol 4-kinase is required for endosomal trafficking and degradation of the EGF receptor [J].

Minogue, S ;

Waugh, MG ;

De Matteis, MA ;

Stephens, DJ ;

Berditchevski, F ;

Hsuan, JJ .

JOURNAL OF CELL SCIENCE, 2006, 119 (03) :571-580

[26] A WORTMANNIN-SENSITIVE PHOSPHATIDYLINOSITOL 4-KINASE THAT REGULATES HORMONE-SENSITIVE POOLS OF INOSITOLPHOSPHOLIPIDS [J].

NAKANISHI, S ;

CATT, KJ ;

BALLA, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (12) :5317-5321

[27] Determining selectivity of phosphoinositide-binding domains [J].

Narayan, Kartik ;

Lemmon, Mark A. .

METHODS, 2006, 39 (02) :122-133

[28] The membrane transport factor TAP/p115 cycles between the Golgi and earlier secretory compartments and contains distinct domains required for its localization and function [J].

Nelson, DS ;

Alvarez, C ;

Gao, YS ;

García-Mata, R ;

Fialkowski, E ;

Sztul, E .

JOURNAL OF CELL BIOLOGY, 1998, 143 (02) :319-331

[29]

Osborne SL, 2001, J CELL SCI, V114, P2501

[30] MOLECULAR TRAPPING OF A FLUORESCENT CERAMIDE ANALOG AT THE GOLGI-APPARATUS OF FIXED CELLS - INTERACTION WITH ENDOGENOUS LIPIDS PROVIDES A TRANS-GOLGI MARKER FOR BOTH LIGHT AND ELECTRON-MICROSCOPY [J].

PAGANO, RE ;

SEPANSKI, MA ;

MARTIN, OC .

JOURNAL OF CELL BIOLOGY, 1989, 109 (05) :2067-2079

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