A microcrystal selection technique in protein crystallization

被引：5

作者：

Han, Q

Lin, SX

机构：

[1] CHU LAVAL,RES CTR,MRC,GRP MOL ENDOCRINOL,QUEBEC CITY,PQ G1V 4G2,CANADA

[2] UNIV LAVAL,QUEBEC CITY,PQ G1V 4G2,CANADA

来源：

JOURNAL OF CRYSTAL GROWTH | 1996年 / 168卷 / 1-4期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0022-0248(96)00352-1

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

The goal of protein crystallization is to obtain high quality single crystals for X-ray diffraction analysis. A new and easy technique was employed to control the number and quality of crystals by eliminating poor microcrystals after the spontaneous nucleation. The process was carried out with two samples: human 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) and hen egg white lysozyme. The present study suggests a useful method for the successful crystal growth of biomacromolecules.

引用

页码：181 / 184

页数：4

共 12 条

[1] CUDNEY B, 1994, ACTA CRYSTALLOGR D, V50, P414, DOI 10.1107/S0907444994002660
[2] CONTROLLING NUCLEATION IN PROTEIN SOLUTIONS
DEMATTEI, RC
FEIGELSON, RS
[J]. JOURNAL OF CRYSTAL GROWTH, 1992, 122 (1-4) : 21 - 30
[3] DUCRUIX A, 1992, CRYSTALLIZATION NUCL, P1
[4] EPITAXIAL-GROWTH OF PROTEIN CRYSTALS ON LIPID LAYERS
EDWARDS, AM
DARST, SA
HEMMING, SA
LI, Y
KORNBERG, RD
[J]. NATURE STRUCTURAL BIOLOGY, 1994, 1 (03): : 195 - 197
[5] HAN Q, 1996, TECHNIQUES PROTEIN C, V7, P361
[6] LIN SX, 1992, J BIOL CHEM, V267, P16182
[7] HETEROGENEOUS AND EPITAXIAL NUCLEATION OF PROTEIN CRYSTALS ON MINERAL SURFACES
MCPHERSON, A
SHLICHTA, P
[J]. SCIENCE, 1988, 239 (4838) : 385 - 387
[8] GROWTH OF PROTEIN CRYSTALS SUSPENDED IN A CLOSED-LOOP THERMOSIPHON
NYCE, TA
ROSENBERGER, F
[J]. JOURNAL OF CRYSTAL GROWTH, 1991, 110 (1-2) : 52 - 59
[9] ROBERT M, 1995, ACTA CRYSTALLOGR D, V50, P496
[10] APPLICATIONS OF THE STREAK SEEDING TECHNIQUE IN PROTEIN CRYSTALLIZATION
STURA, EA
WILSON, IA
[J]. JOURNAL OF CRYSTAL GROWTH, 1991, 110 (1-2) : 270 - 282

← 1 2 →