A microcrystal selection technique in protein crystallization

被引：5

作者：

Han, Q

Lin, SX

机构：

[1] CHU LAVAL,RES CTR,MRC,GRP MOL ENDOCRINOL,QUEBEC CITY,PQ G1V 4G2,CANADA

[2] UNIV LAVAL,QUEBEC CITY,PQ G1V 4G2,CANADA

来源：

JOURNAL OF CRYSTAL GROWTH | 1996年 / 168卷 / 1-4期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0022-0248(96)00352-1

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

The goal of protein crystallization is to obtain high quality single crystals for X-ray diffraction analysis. A new and easy technique was employed to control the number and quality of crystals by eliminating poor microcrystals after the spontaneous nucleation. The process was carried out with two samples: human 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) and hen egg white lysozyme. The present study suggests a useful method for the successful crystal growth of biomacromolecules.

引用

页码：181 / 184

页数：4