Coupling Sensitive Nucleic Acid Amplification with Commercial Pregnancy Test Strips

被引：147

作者：

Du, Yan ^{[1
,2
]}

Pothukuchy, Arti ^{[1
,2
]}

Gollihar, Jimmy D. ^{[1
,2
]}

Nourani, Armin ^{[1
,2
]}

Li, Bingling ^{[3
]}

Ellington, Andrew D. ^{[1
,2
]}

机构：

[1] Univ Texas Austin, Inst Cellular & Mol Biol, Ctr Syst & Synthet Biol, Austin, TX 78712 USA

[2] Univ Texas Austin, Dept Chem, Austin, TX 78712 USA

[3] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electrocanlyt Chem, Jilin, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2017年 / 56卷 / 04期

基金：

美国国家卫生研究院;

关键词：

human chorionic gonadotropin; loop-mediated isothermal amplification (LAMP); nucleic acid amplification; pregnancy test strips; SNAP protein; LATERAL FLOW DIPSTICK; ISOTHERMAL AMPLIFICATION; MEDICAL DIAGNOSTICS; STRANDED-DNA; GLUCOSE; NANOTECHNOLOGIES; BIOSENSORS;

D O I：

10.1002/anie.201609108

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The detection of nucleic acid biomarkers for point-of-care (POC) diagnostics is currently limited by technical complexity, cost, and time constraints. To overcome these shortcomings, we have combined loop-mediated isothermal amplification (LAMP), programmable toehold-mediated strand-exchange signal transduction, and standard pregnancy test strips. The incorporation of an engineered hCG-SNAP fusion reporter protein (human chorionic gonadotropin-O-6-alkylguanine-DNA alkyltransferase) led to LAMP-to-hCG signal transduction on low-cost, commercially available pregnancy test strips. Our assay reliably detected as few as 20 copies of Ebola virus templates in both human serum and saliva and could be adapted to distinguish a common melanoma-associated SNP allele (BRAF V600E) from the wild-type sequence. The methods described are completely generalizable to many nucleic acid biomarkers, and could be adapted to provide POC diagnostics for a range of pathogens.

引用

页码：992 / 996

页数：5

共 24 条

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