An F-box gene linked to the self-incompatibility (S) locus of Antirrhinum is expressed specifically in pollen and tapetum

被引:332
作者
Lai, Z
Ma, WS
Han, B
Liang, LZ
Zhang, YS
Hong, GF
Xue, YB [1 ]
机构
[1] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100080, Peoples R China
[2] Chinese Acad Sci, Natl Ctr Gene Res, Shanghai 200233, Peoples R China
基金
中国国家自然科学基金;
关键词
Antirrhinum; F-box; gametophyte; self-incompatibility; S locus structure;
D O I
10.1023/A:1016050018779
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In many flowering plants, self-fertilization is prevented by an intraspecific reproductive barrier known as self-incompatibility (SI), that, in most cases, is controlled by a single multiallelic S locus. So far, the only known S locus product in self-incompatible species from the Solanaceae, Scrophulariaceae and Rosaceae is a class of ribonucleases called S RNases. Molecular and transgenic analyses have shown that S RNases are responsible for pollen rejection by the pistil but have no role in pollen expression of SI, which appears to be mediated by a gene called the pollen self-incompatibility or Sp gene. To identify possible candidates for this gene, we investigated the genomic structure of the S locus in Antirrhinum, a member of the Scrophulariaceae. A novel F-box gene, AhSLF-S-2, encoded by the S-2 allele, with the expected features of the Sp gene was identified. AhSLF-S-2 is located 9 kb downstream of S-2 RNase gene and encodes a polypeptide of 376 amino acids with a conserved F-box domain in its amino-terminal part. Hypothetical genes homologous to AhSLF-S-2 are apparent in the sequenced genomic DNA of Arabidopsis and rice. Together, they define a large gene family, named SLF (S locus F-box) family. AhSLF-S-2 is highly polymorphic and is specifically expressed in tapetum, microspores and pollen grains in an allele-specific manner. The possibility that Sp encodes an F-box protein and the implications of this for the operation of self-incompatibility are discussed.
引用
收藏
页码:29 / 42
页数:14
相关论文
共 62 条
  • [1] SELF-INCOMPATIBILITY IN PETUNIA-INFLATA - ISOLATION AND CHARACTERIZATION OF CDNAS ENCODING 3 S-ALLELE-ASSOCIATED PROTEINS
    AI, YJ
    SINGH, A
    COLEMAN, CE
    IOERGER, TR
    KHEYRPOUR, A
    KAO, TH
    [J]. SEXUAL PLANT REPRODUCTION, 1990, 3 (02): : 130 - 138
  • [2] SEQUENCE VARIABILITY OF 3 ALLELES OF THE SELF-INCOMPATIBILITY GENE OF NICOTIANA-ALATA
    ANDERSON, MA
    MCFADDEN, GI
    BERNATZKY, R
    ATKINSON, A
    ORPIN, T
    DEDMAN, H
    TREGEAR, G
    FERNLEY, R
    CLARKE, AE
    [J]. PLANT CELL, 1989, 1 (05) : 483 - 491
  • [3] CLONING OF CDNA FOR A STYLAR GLYCOPROTEIN ASSOCIATED WITH EXPRESSION OF SELF-INCOMPATIBILITY IN NICOTIANA-ALATA
    ANDERSON, MA
    CORNISH, EC
    MAU, SL
    WILLIAMS, EG
    HOGGART, R
    ATKINSON, A
    BONIG, I
    GREGO, B
    SIMPSON, R
    ROCHE, PJ
    HALEY, JD
    PENSCHOW, JD
    NIALL, HD
    TREGEAR, GW
    COGHLAN, JP
    CRAWFORD, RJ
    CLARKE, AE
    [J]. NATURE, 1986, 321 (6065) : 38 - 44
  • [4] SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box
    Bai, C
    Sen, P
    Hofmann, K
    Ma, L
    Goebl, M
    Harper, JW
    Elledge, SJ
    [J]. CELL, 1996, 86 (02) : 263 - 274
  • [5] BANKIER AT, 1987, METHOD ENZYMOL, V155, P51
  • [6] NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS
    BENNETT, MD
    LEITCH, IJ
    [J]. ANNALS OF BOTANY, 1995, 76 (02) : 113 - 176
  • [7] A new DNA sequence assembly program
    Bonfield, JK
    Smith, KF
    Staden, R
    [J]. NUCLEIC ACIDS RESEARCH, 1995, 23 (24) : 4992 - 4999
  • [8] BREWBAKER JL, 1960, GENETICS, V45, P699
  • [9] CDNA CLONING AND MOLECULAR ANALYSIS OF 2 SELF-INCOMPATIBILITY ALLELES FROM APPLE
    BROOTHAERTS, W
    JANSSENS, GA
    PROOST, P
    BROEKAERT, WF
    [J]. PLANT MOLECULAR BIOLOGY, 1995, 27 (03) : 499 - 511
  • [10] BURGE C, 1997, J MOL BIOL, V268, P28