A critical and integrated view of the yeast interactome

被引:9
作者
Cornell, M
Paton, NW
Oliver-, SG
机构
[1] Univ Manchester, Dept Comp Sci, Manchester M13 9PL, Lancs, England
[2] Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England
来源
COMPARATIVE AND FUNCTIONAL GENOMICS | 2004年 / 5卷 / 05期
关键词
Saccharomyces cerevisiae; protein interactions; data integration;
D O I
10.1002/cfg.412
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Global studies of protein-protein interactions are crucial to both elucidating gene function and producing an integrated view of the workings of living cells. High-throughput studies of the yeast interactome have been performed using both genetic and biochemical screens. Despite their size, the overlap between these experimental datasets is very limited. This could be due to each approach sampling only a small fraction of the total interactome. Alternatively, a large proportion of the data from these screens may represent false-positive interactions. We have used the Genome Information Management System (GIMS) to integrate interactome datasets with transcriptome and protein annotation data and have found significant evidence that the proportion of false-positive results is high. Not all high-throughput datasets are similarly contaminated, and the tandem affinity purification (TAP) approach appears to yield a high proportion of reliable interactions for which corroborating evidence is available. From our integrative analyses, we have generated a set of verified interactome data for yeast. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码:382 / 402
页数:21
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