The nad4L-orf25 gene cluster is conserved and expressed in sugar beet mitochondria

被引:13
作者
Kubo, T [1 ]
Yamamoto, MP [1 ]
Mikami, T [1 ]
机构
[1] Hokkaido Univ, Fac Agr, Lab Genet Engn, Sapporo, Hokkaido 0608589, Japan
关键词
nad4L-orf25; linkage; RNA editing; sugar beet; mitochondria;
D O I
10.1007/s001220050029
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
We have found that a gene coding for NADH dehydrogenase subunit 4L and a presumed gene, orf25, are Linked and co-transcribed with each other in sugar beet mitochondria. Ten and twelve C-to-U editing events were observed in the mRNAs of nad4L and orf25, respectively; the amino-acid sequence specified after editing is better-conserved in comparison with the homologues of other organisms. It is interesting to note that the translation initiation codon of nad4L is created by editing. The conservation of the nad4L-orf25 linkage was examined by PCR-amplification of the intergenic region. We obtained successful PCR products from five dicots (spinach, apple, snapdragon, petunia and tobacco) and two monocots (tulip and pineapple), but not in two poaceous plants, rice and maize. The intergenic region, when present, was found to be well-conserved in its sequence, suggesting a monophyletic origin of this linkage. Our result, together with previous reports of Arabidopsis and four poaceous species, favour the argument that the nad4L-orf25 linkage is conserved throughout angiosperms except in the Poaceae.
引用
收藏
页码:214 / 220
页数:7
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