Using Aptamer-Conjugated Fluorescence Resonance Energy Transfer Nanoparticles for Multiplexed Cancer Cell Monitoring

被引:137
作者
Chen, Xiaolan [1 ,2 ,3 ,4 ]
Estevez, M. -Carmen [1 ,2 ,3 ]
Zhu, Zhi [1 ,2 ,3 ]
Huang, Yu-Fen [1 ,2 ,3 ]
Chen, Yan [1 ,2 ,3 ]
Wang, Lin [5 ]
Tan, Weihong [1 ,2 ,3 ]
机构
[1] Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA
[2] Univ Florida, Dept Physiol & Funct Genom, Shands Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA
[3] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA
[4] Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China
[5] Eli Lilly & Co, Lilly Res Labs, Indianapolis, IN 46285 USA
关键词
SILICA NANOPARTICLES; EXPONENTIAL ENRICHMENT; MOLECULAR RECOGNITION; SYSTEMATIC EVOLUTION; LIGANDS; SELECTION; DIAGNOSTICS; COLLECTION; MICROARRAY; BIOMARKERS;
D O I
10.1021/ac9011073
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
To facilitate the selection of effective therapeutic pathways and improve clinical outcomes, sensitive and simultaneous diagnosis of multiple trace biomarkers or cancer cells from complex living samples is particularly critical in the early stages of tumor development. To achieve this, we have combined the selectivity and affinity of aptamers with the spectroscopic advantages of fluorescence resonance energy transfer (FRET) nanoparticles (NPs). This has produced an aptamer-conjugated FRET NP assay that performs simultaneous multiplexed monitoring of cancer cells with the desired degree of sensitivity and selectivity. First, by changing the doping ratio of three different dyes, the FRET-mediated emission signatures could be tuned such that the nanoparticles would exhibit multiple colors upon excitation with a single wavelength. These FRET nanoparticles were then modified by a few aptamers specific for different cancer cell lines, in this case, T-cell leukemia and B-cell lymphoma. As, a result, simultaneous and sensitive detection of multiple cancer cell targets was achieved. Therefore, our aptamer-conjugated FRET NPs are highly promising for potential applications in the sensitive monitoring of multiple cancer cells for biomedical research and medical diagnostics.
引用
收藏
页码:7009 / 7014
页数:6
相关论文
共 29 条
[1]  
Brody E N, 2000, J Biotechnol, V74, P5, DOI 10.1016/S1389-0352(99)00004-5
[2]   Fluorescent Silica Nanoparticles with Efficient Urinary Excretion for Nanomedicine [J].
Burns, Andrew A. ;
Vider, Jelena ;
Ow, Hooisweng ;
Herz, Erik ;
Penate-Medina, Oula ;
Baumgart, Martin ;
Larson, Steven M. ;
Wiesner, Ulrich ;
Bradbury, Michelle .
NANO LETTERS, 2009, 9 (01) :442-448
[3]   Preparation and fluoroimmunoassay application of new red-region fluorescent silica nanoparticles [J].
Chen, Xiao-Lan ;
Zou, Jian-Li ;
Zhao, Ting-Ting ;
Li, Zhong-Bao .
JOURNAL OF FLUORESCENCE, 2007, 17 (02) :235-241
[4]   A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment [J].
Daniels, DA ;
Chen, H ;
Hicke, BJ ;
Swiderek, KM ;
Gold, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (26) :15416-15421
[5]   INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].
ELLINGTON, AD ;
SZOSTAK, JW .
NATURE, 1990, 346 (6287) :818-822
[6]  
Estévez MC, 2009, NANO RES, V2, P448, DOI [10.1007/s12274-009-9041-8, 10.1007/S12274-009-9041-8]
[7]  
Famulok M, 1999, CURR TOP MICROBIOL, V243, P123
[8]   Multiplexed proximity ligation assays to profile putative plasma biomarkers relevant to pancreatic and ovarian cancer [J].
Fredriksson, Simon ;
Horecka, Joe ;
Brustugun, Odd Terje ;
Schlingemann, Joerg ;
Koong, Albert C. ;
Tibshirani, Rob ;
Davis, Ronald W. .
CLINICAL CHEMISTRY, 2008, 54 (03) :582-589
[9]   New method to prepare very stable and biocompatible fluorescent silica nanoparticles [J].
Ha, Shin-Woo ;
Camalier, Corinne E. ;
Beck, George R., Jr. ;
Lee, Jin-Kyu .
CHEMICAL COMMUNICATIONS, 2009, (20) :2881-2883
[10]   Aptamer-conjugated nanoparticles for selective collection and detection of cancer cells [J].
Herr, Joshua K. ;
Smith, Joshua E. ;
Medley, Colin D. ;
Shangguan, Dihua ;
Tan, Weihong .
ANALYTICAL CHEMISTRY, 2006, 78 (09) :2918-2924