Detection of norovirus antigens from recombinant virus-like particles and stool samples by a commercial norovirus enzyme-linked immunosorbent assay kit

被引：24

作者：

Okitsu-Negishi, Shoko

Kame, Michio

Shimizu, Yuko

Phan, Tung Gia

Tomaru, Takeshi

Kamijo, Shigenori

Sato, Takashi

Yagyu, Fumihiro

Mueller, Werner E. G.

Ushijima, Hiroshi

机构：

[1] Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan

[2] Nippon Becton Dickinson Co, Tokyo, Japan

[3] Johannes Gutenberg Univ Mainz, Inst Physiol Chem, Angew Mol Biol Abt, D-6500 Mainz, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 10期

关键词：

D O I：

10.1128/JCM.01373-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The commercial norovirus enzyme-linked immunosorbent assay kit was evaluated for its reactivity to recombinant virus-like particles and the detection of natural viruses from stool samples of Japanese infants and children with sporadic acute gastroenteritis compared to reverse transcription-PCR. The kit had a sensitivity of 76.3% and a specificity of 94.9%. Our results clearly indicated that the kit allows the detection of the most prevalent genotype, GII/4. In order to increase the sensitivity of the kit, the reactivity with norovirus of GII/3 and GII/6 genotypes needs to be improved.

引用

页码：3784 / 3786

页数：3

共 21 条

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