Detection of norovirus antigens from recombinant virus-like particles and stool samples by a commercial norovirus enzyme-linked immunosorbent assay kit
被引:24
作者:
Okitsu-Negishi, Shoko
论文数: 0引用数: 0
h-index: 0
机构:Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
Okitsu-Negishi, Shoko
Kame, Michio
论文数: 0引用数: 0
h-index: 0
机构:Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
Kame, Michio
Shimizu, Yuko
论文数: 0引用数: 0
h-index: 0
机构:Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
Shimizu, Yuko
Phan, Tung Gia
论文数: 0引用数: 0
h-index: 0
机构:Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
Phan, Tung Gia
Tomaru, Takeshi
论文数: 0引用数: 0
h-index: 0
机构:Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
Tomaru, Takeshi
Kamijo, Shigenori
论文数: 0引用数: 0
h-index: 0
机构:Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
Kamijo, Shigenori
Sato, Takashi
论文数: 0引用数: 0
h-index: 0
机构:Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
Sato, Takashi
Yagyu, Fumihiro
论文数: 0引用数: 0
h-index: 0
机构:Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
Yagyu, Fumihiro
Mueller, Werner E. G.
论文数: 0引用数: 0
h-index: 0
机构:Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
Mueller, Werner E. G.
Ushijima, Hiroshi
论文数: 0引用数: 0
h-index: 0
机构:Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
Ushijima, Hiroshi
机构:
[1] Univ Tokyo, Dept Dev Med Sci, Sch Int Hlth, Grad Sch Med,Bunkyo Ku, Tokyo 1130033, Japan
The commercial norovirus enzyme-linked immunosorbent assay kit was evaluated for its reactivity to recombinant virus-like particles and the detection of natural viruses from stool samples of Japanese infants and children with sporadic acute gastroenteritis compared to reverse transcription-PCR. The kit had a sensitivity of 76.3% and a specificity of 94.9%. Our results clearly indicated that the kit allows the detection of the most prevalent genotype, GII/4. In order to increase the sensitivity of the kit, the reactivity with norovirus of GII/3 and GII/6 genotypes needs to be improved.
引用
收藏
页码:3784 / 3786
页数:3
相关论文
共 21 条
[21]
Yan YN, 2000, J GLOB MARK, V14, P37, DOI 10.1300/J042v14n01_03