A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure

被引：104

作者：

Antonis, Adriaan F. G.

Bruschke, Christianne J. M.

Rueda, Paloma

Maranga, Luis

Casal, J. Ignacio

Vela, Carmen

Hilgers, Luuk A. Th.

Belt, Peter B. G. M.

Weerdmeester, Klaas

Carrondo, Manuel J. T.

Langeveld, Jan P. M.

机构：

[1] Anim Sci Grp, NL-8200 AB Lelystad, Netherlands

[2] Inmunol & Genet Aplicada SA, Madrid 28037, Spain

[3] IBET, P-2780 Oeiras, Portugal

[4] CoVaccine BV, NL-3583 HE Utrecht, Netherlands

来源：

VACCINE | 2006年 / 24卷 / 26期

关键词：

porcine parvovirus; virus-like particles; vaccine; recombinant;

D O I：

10.1016/j.vaccine.2006.03.089

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A novel vaccine against porcine parvovirus (PPV), composed of recombinant virus-like particles (PPV-VLPs) produced with the baculovirus expression vector system (BEVS) at industrial scale, was tested for its immunogenicity and protective potency. A formulation of submicrogram amounts of PPV-VLPs in a water-in-mineral oil adjuvant evoked high serum antibody titres in both guinea pigs, used as reference model, and target species, pigs. A single immunisation with 0.7 mu g of this antigen yielded complete foetal protection against PPV infection after challenge with a virulent strain of this virus. Furthermore, also in the presence of mild adjuvants the protective action of these PPV-VLPs is excellent. This recombinant subunit vaccine overcomes some of the drawbacks of classical PPV vaccines. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：5481 / 5490

页数：10

共 42 条

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