Immobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices

被引：33

作者：

Alkan, S

Toppare, L ^{[1
]}

Yagci, Y

Hepuzer, Y

机构：

[1] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey

[2] Istanbul Tech Univ, Dept Chem, TR-80626 Istanbul, Turkey

来源：

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 1999年 / 10卷 / 12期

关键词：

immobilization; invertase; poly(methylmethacrylate); electropolymerization; polypyrrole;

D O I：

10.1163/156856299X00036

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Immobilization of invertase in thiophene-capped poly(methylmethacrylate)/polypyrrole matrices was achieved by constant potential electrolysis using different supporting electrolytes. Optimum reaction conditions such as substrate concentration, temperature, and pH for the enzyme electrodes were determined. The temperature and pH were found to be 60 degrees C and 4.8, respectively. The effect of supporting electrolyte on the enzyme activity revealed that SDS was the best in the immobilization procedure. Michaelis-Menten constant and the maximum reaction rate in PMMA/PPy matrices were of the order of that of pristine polypyrrole. However, in terms of repeated use, the copolymer matrices were superior to polypyrrole.

引用

页码：1223 / 1235

页数：13

共 20 条

[1]

ALKAN S, 1999, UNPUB J POLYM SCI CH

[2] ELECTROCHEMICAL IMMOBILIZATION OF ENZYMES .2. GLUCOSE-OXIDASE IMMOBILIZED IN POLY-N-METHYLPYRROLE [J].