Isolation and identification of swine influenza recombinant A/Swine/Shandong/1/2003(H9N2) virus

被引：151

作者：

Xu, CT ^{[1
]}

Fan, WX ^{[1
]}

Wei, R ^{[1
]}

Zhao, HK ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Anim Sci, Tainan 271018, Shandong, Peoples R China

来源：

MICROBES AND INFECTION | 2004年 / 6卷 / 10期

关键词：

swine influenza viruses; H9N2; subtype; reassortment;

D O I：

10.1016/j.micinf.2004.04.015

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Ten influenza virus isolates were obtained from infected pigs from different places in Shandong province showing clinical symptoms from October 2002 to January 2003. All 10 isolates were identified in China's National Influenza Research Center as influenza A virus of H9N2 subtype. The complete genome of one isolate, designated A/Swine/Shandong/1/2003(H9N2), was sequenced and compared with sequences available in GenBank. The results of analyses indicated that the sequence of A/Swine/Shandong/1/2003(H9N2) was similar to those of several chicken influenza viruses and duck influenza viruses recently prevalent in South China. According to phylogenetic analysis of the complete gene sequences, A/Swine/Shandong/1/2003(H9N2) possibly originated from the reassortment of chicken influenza viruses and duck influenza viruses. It was found that the amino acid sequence at the HA cleavage site in Sw/SD/1/2003 is R-S-L-R-G, differing clearly from that of other H9N2 subtype isolates of swine influenza and avian influenza, which is R-S-S-R-G. (C) 2004 Elsevier SAS. All rights reserved.

引用

页码：919 / 925

页数：7