Microbial Antagonists of Escherichia coli O157:H7 on Fresh-Cut Lettuce and Spinach

被引:28
作者
Johnston, Michael A. [1 ]
Harrison, Mark A. [1 ]
Morrow, Ruth A. [1 ]
机构
[1] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA
关键词
LACTIC-ACID BACTERIA; LISTERIA-MONOCYTOGENES; O157-H7; PRODUCE; GROWTH; MICROFLORA; PATHOGENS; ANTIMICROBIALS; INHIBITION; SURVIVAL;
D O I
10.4315/0362-028X-72.7.1569
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fresh-cut lettuce and spinach can become contaminated with pathogens at numerous points from the field to the retail market. Natural microflora present off fresh produce may hell) reduce the pathogen load. The objective of this Study was to isolate natural microflora front fresh-cut iceberg lettuce and baby spinach and to determine whether these bacteria were antagonistic toward Escherichia coli H157:O7. Samples were collected under conditions that mimicked actual practices between production and retail sale. Evidence of naturally Occurring microorganisms on fresh lettuce (295 isolates) and spinach (200 isolates) and of possible antagonistic activity toward E. coli O157:H7 was documented. Inhibitory activity by several isolates was due to either acid production or antimicrobial peptides. Bacteria with inhibitory activity were isolated from every step in the processing and handling of the fresh-cut iceberg lettuce and baby spinach.
引用
收藏
页码:1569 / 1575
页数:7
相关论文
共 26 条
[1]  
ADAMS MR, 1998, FOOD MICROBIOL, V59, P1999
[2]   Differences in attachment of Salmonella enterica serovars and Escherichia coli O157:H7 to alfalfa sprouts [J].
Barak, JD ;
Whitehand, LC ;
Charkowski, AO .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (10) :4758-4763
[3]   Pathogenic microorganisms associated with fresh produce [J].
Beuchat, LR .
JOURNAL OF FOOD PROTECTION, 1996, 59 (02) :204-216
[4]   Produce handling and processing practices [J].
Beuchat, LR ;
Ryu, JH .
EMERGING INFECTIOUS DISEASES, 1997, 3 (04) :459-465
[5]  
Brackett R.E., 1994, MINIMALLY PROCESSED, P269, DOI [10.1007/978-1-4615-2393-2_7, DOI 10.1007/978-1-4615-2393-2_7]
[6]   Protective cultures inhibit growth of Listeria monocytogenes and Escherichia coli O157:H7 in cooked, sliced, vacuum- and gas-packaged meat [J].
Bredholt, S ;
Nesbakken, T ;
Holck, A .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 53 (01) :43-52
[7]  
*CDCP, 2006, ONG MULT STAT OUTBR
[8]  
Centers for Disease Control and Prevention, 2006, UPD MULT STAT OUTBR
[9]  
Cherry JP, 1999, FOOD TECHNOL-CHICAGO, V53, P54
[10]   Escherichia coli O157:H7 survival and growth on lettuce is altered by the presence of epiphytic bacteria [J].
Cooley, Michael B. ;
Chao, Diana ;
Mandrell, Robert E. .
JOURNAL OF FOOD PROTECTION, 2006, 69 (10) :2329-2335