Microbial Antagonists of Escherichia coli O157:H7 on Fresh-Cut Lettuce and Spinach

被引:28
作者
Johnston, Michael A. [1 ]
Harrison, Mark A. [1 ]
Morrow, Ruth A. [1 ]
机构
[1] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA
关键词
LACTIC-ACID BACTERIA; LISTERIA-MONOCYTOGENES; O157-H7; PRODUCE; GROWTH; MICROFLORA; PATHOGENS; ANTIMICROBIALS; INHIBITION; SURVIVAL;
D O I
10.4315/0362-028X-72.7.1569
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Fresh-cut lettuce and spinach can become contaminated with pathogens at numerous points from the field to the retail market. Natural microflora present off fresh produce may hell) reduce the pathogen load. The objective of this Study was to isolate natural microflora front fresh-cut iceberg lettuce and baby spinach and to determine whether these bacteria were antagonistic toward Escherichia coli H157:O7. Samples were collected under conditions that mimicked actual practices between production and retail sale. Evidence of naturally Occurring microorganisms on fresh lettuce (295 isolates) and spinach (200 isolates) and of possible antagonistic activity toward E. coli O157:H7 was documented. Inhibitory activity by several isolates was due to either acid production or antimicrobial peptides. Bacteria with inhibitory activity were isolated from every step in the processing and handling of the fresh-cut iceberg lettuce and baby spinach.
引用
收藏
页码:1569 / 1575
页数:7
相关论文
共 26 条
[11]  
DOERING HJ, USE SYSTEMS AP UNPUB
[12]   MICROBIAL INHIBITION BY AN ISOLATE OF PEDIOCOCCUS FROM CUCUMBER BRINES [J].
FLEMING, HP ;
ETCHELLS, JL ;
COSTILOW, RN .
APPLIED MICROBIOLOGY, 1975, 30 (06) :1040-1042
[13]   Effects of the indigenous microflora of minimally processed lettuce on the survival and growth of Listeria innocua [J].
Francis, GA ;
O'Beirne, D .
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 1998, 33 (05) :477-488
[14]  
Harrigan W., 1976, Laboratory methods in food and dairy microbiology, P47
[15]  
Harris L.J., 2003, Compr. Rev. Food Sci. Food Saf, V2, P78, DOI DOI 10.1111/J.1541-4337.2003.TB00031.X
[16]   Interactions of Escherichia coli O157:147, Salmonella typhimurium and Listeria monocytogenes plants cultivated in a gnotobiotic system [J].
Jablasone, J ;
Warriner, K ;
Griffiths, M .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2005, 99 (01) :7-18
[17]   Biological control of postharvest decays of apple can prevent growth of Escherichia coli O157:H7 in apple wounds [J].
Janisiewicz, WJ ;
Conway, WS ;
Leverentz, B .
JOURNAL OF FOOD PROTECTION, 1999, 62 (12) :1372-1375
[18]  
Kader A.A., 1992, PUBLICATION U CALIFO
[19]   INHIBITION OF FOOD-BORNE BACTERIAL PATHOGENS BY BACTERIOCINS FROM LACTIC-ACID BACTERIA ISOLATED FROM MEAT [J].
LEWUS, CB ;
KAISER, A ;
MONTVILLE, TJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (06) :1683-1688
[20]   Analysis of native microflora and selection of strains antagonistic to human pathogens on fresh produce [J].
Liao, CS ;
Fett, WF .
JOURNAL OF FOOD PROTECTION, 2001, 64 (08) :1110-1115