Analysis of highly polar metabolites in human plasma by ultra-performance hydrophilic interaction liquid chromatography coupled with quadrupole-time of flight mass spectrometry

被引：58

作者：

Cai, Xiaoming ^{[1
]}

Zou, Lijuan ^{[1
,2
]}

Dong, Jun ^{[1
]}

Zhao, Lili ^{[2
]}

Wang, Yuanyuan ^{[2
]}

Xu, Qing ^{[1
]}

Xue, Xingya ^{[1
]}

Zhang, Xiuli ^{[1
]}

Liang, Xinmiao ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China

[2] Dalian Med Univ, Hosp 2, Dalian 116023, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2009年 / 650卷 / 01期

关键词：

Hydrophilic interaction liquid chromatography; Plasma; Metabonomics; Highly polar metabolites; PROTEIN PRECIPITATION; RAT URINE; METABONOMICS; SEPARATION; SPECTROSCOPY; AGE;

D O I：

10.1016/j.aca.2009.01.054

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Highly polar metabolites are abundant in human plasma. Analysis of these compounds by standard reverse-phase chromatography is difficult. Highly polar metabolites were extracted by a two-step method. They were analyzed by ultra-performance hydrophilic interaction chromatography (HILIC) coupled to quadrupole-time of flight mass spectrometer detection technology. The middle and low polar fraction were also analyzed using a standard C18 column. Data were extracted by Markerlynx, and analyzed by multivariate statistical methods to discriminate human plasma samples by sex and age. The results not only showed good classification of samples by sex and age, but also revealed new potential biomarkers not revealed by standard RPLC/MS analysis. This study showed that HILIC is an effective technique to analyze highly polar metabolites for metabonomic studies. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：10 / 15

页数：6

共 26 条

[1] HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS [J].

ALPERT, AJ .

JOURNAL OF CHROMATOGRAPHY, 1990, 499 :177-196

[2] Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry [J].

Bajad, Sunil U. ;

Lu, Wenyun ;

Kimball, Elizabeth H. ;

Yuan, Jie ;

Peterson, Celeste ;

Rabinowitz, Joshua D. .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1125 (01) :76-88

[3] Hydrophilic interaction chromatography for mass spectrometric metabonomic studies of urine [J].

Cubbon, Simon ;

Bradbury, Timothy ;

Wilson, Julie ;

Thomas-Oates, Jane .

ANALYTICAL CHEMISTRY, 2007, 79 (23) :8911-8918

[4]

Drexler Dieter M, 2004, Drug Discov Today Technol, V1, P17, DOI 10.1016/j.ddtec.2004.08.010

[5] Hydrophilic interaction chromatography coupled to nuclear magnetic resonance spectroscopy and mass spectroscopy - A new approach for the separation and identification of extremely polar analytes in bodyfluids [J].

Godejohann, Markus .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1156 (1-2) :87-93

[6] A metabonomic study of strain- and age-related differences in the Zucker rat [J].

Granger, Jennifer H. ;

Williams, Rebecca ;

Lenz, Eva M. ;

Plumb, Robert S. ;

Stumpf, Chris L. ;

Wilson, Ian D. .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2007, 21 (13) :2039-2045

[7] Retention behavior of small polar compounds on polar stationary phases in hydrophilic interaction chromatography [J].

Guo, Y ;

Gaiki, S .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1074 (1-2) :71-80

[8] Metabolic fingerprinting of rat urine by LC/MS Part 2. Data pretreatment methods for handling of complex data [J].

Idborg, H ;

Zamani, L ;

Edlund, PO ;

Schuppe-Koistinen, I ;

Jacobsson, SP .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 828 (1-2) :14-20

[9] Metabolic fingerprinting of rat urine by LC/MS Part 1. Analysis by hydrophilic interaction liquid chromatography-electrospray ionization mass spectrometry [J].

Idborg, H ;

Zamani, L ;

Edlund, PO ;

Schuppe-Koistinen, I ;

Jacobsson, SP .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 828 (1-2) :9-13

[10] A comprehensive urinary metabolomic approach for identifying kidney cancer [J].

Kind, Tobias ;

Tolstikov, Vladimir ;

Fiehn, Oliver ;

Weiss, Robert H. .

ANALYTICAL BIOCHEMISTRY, 2007, 363 (02) :185-195

← 1 2 3 →