Inhibition of MAP kinase blocks insulin-mediated DNA synthesis and transcriptional activation of c-fos by Elk-1 in vascular smooth muscle cells

被引：47

作者：

Xi, XP

Graf, K

Goetze, S

Hsueh, WA

Law, RE

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,DIV ENDOCRINOL DIABET & HYPERTENS,LOS ANGELES,CA 90024

[2] VIRCHOW KLINIKUM HU BERLIN,DEPT MED CARDIOL,BERLIN,GERMANY

[3] GERMAN HEART INST,BERLIN,GERMANY

来源：

FEBS LETTERS | 1997年 / 417卷 / 03期

关键词：

vascular smooth muscle cell; insulin; c-fos; serum response element; Elk-1; mitogen-activated protein kinase;

D O I：

10.1016/S0014-5793(97)01303-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin-stimulated DNA synthesis, MAP kinase (MAPK) activity and c-fos expression in vascular smooth muscle cells (VSMCs) was blocked by the MAPK inhibitor PD 98059. Regulation of c-fos expression by the transcription factor Elk-1 at the serum response element (SRE) is dependent on its phosphorylation by MAPK, PD 98059 also suppressed insulin-induced Elk-1 transcriptional activity through the SRE. These data show that MAPK plays a critical role in both insulin-mediated growth and Elk-1-dependent induction of c-fos in VSMCs. (C) 1997 Federation of European Biochemical Societies.

引用

页码：283 / 286

页数：4

共 29 条

[1] BINDING AND BIOLOGICAL EFFECTS OF INSULIN, INSULIN ANALOGS AND INSULIN-LIKE GROWTH-FACTORS IN RAT AORTIC SMOOTH-MUSCLE CELLS - COMPARISON OF MAXIMAL GROWTH-PROMOTING ACTIVITIES [J].