Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography

被引：31

作者：

Chen, F

Lu, HT

Jiang, Y

机构：

[1] Univ Hong Kong, Dept Bot, Hong Kong, Hong Kong, Peoples R China

[2] Hong Kong Baptist Univ, Dept Biol, Kowloon, Hong Kong, Peoples R China

[3] Laiyang Agr Coll, Dept Basic Courses, Shandong 265200, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1040卷 / 02期

关键词：

counter-current chromatography; Paeonia lactiflora; pharmaceutical analysis; plant materials; preparative chromatography; paeoniflorin;

D O I：

10.1016/j.chroma.2004.04.023

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography was successfully used for the first time for the preparative separation and purification of paeoniflorin from the Chinese medicinal plant Paeonia lactiflora Pall. using a two-phase solvent system composed of n-butanol-ethyl acetate-water (1:4:5, v/v) in a single run. From 160 mg of the crude sample containing 22.0% paeoniflorin, 33.2 mg of paeoniflorin was yielded at 98.2% purity as determined by HPLC analysis. The recovery of paeoniflorin was 94.3%. (C) 2004 Elsevier B.V. All rights reserved.

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页码：205 / 208

页数：4

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