Separation of andrographolide and neoandrographolide from the leaves of Andrographis paniculata using high-speed counter-current chromatography

被引：56

作者：

Du, QZ ^{[1
]}

Jerz, G

Winterhalter, P

机构：

[1] Hangzhou Univ Commerce, Inst Food & Biol Engn, Hangzhou 310035, Peoples R China

[2] Tech Univ Carolo Wilhelmina Braunschweig, Inst Food Chem, DE-38106 Braunschweig, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 984卷 / 01期

关键词：

counter-current chromatography; Andrographis paniculata; plant material; andrographolide; neoandrographolide; diterpenes;

D O I：

10.1016/S0021-9673(02)01831-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The bioactive diterpenes andrographolide and neoandrographolide from the leaves of Andrographis paniculata NEES (Acanthaceae) were successfully separated by counter-current chromatography. A single 280-min separation yielded 189 mg of 99.9% andrographolide and 9.5 mg of 98.5% neoandrographolide applying water-methanol-ethyl acetate-n-hexane (2.5:2.5:4:1) solvent system. Structure confirmation was done by electrospray MS, one-dimensional NMR experiments, circular dichroism, optical rotation dispersion, and specific optical rotation [alpha](D). (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：147 / 151

页数：5

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