Structural study on lipid A and the O-specific polysaccharide of the lipopolysaccharide from a clinical isolate of Bacteroides vulgatus from a patient with Crohn's disease

被引:19
作者
Hashimoto, M
Kirikae, F
Dohi, T
Adachi, S
Kusumoto, S
Suda, Y
Fujita, T
Naoki, H
Kirikae, T [1 ]
机构
[1] Int Med Ctr Japan, Res Inst, Shinjuku Ku, Tokyo 1628655, Japan
[2] Asahi Univ, Sch Dent, Dept Oral Microbiol, Gifu, Japan
[3] Osaka Univ, Grad Sch Sci, Suita, Osaka 565, Japan
[4] Hyogo Med Univ, Dept Bacteriol, Nishinomiya, Hyogo, Japan
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 2002年 / 269卷 / 15期
关键词
Bacteroides vulgatus; fast-atom-bombardment tandem mass spectrometry; lipopolysaccharide; MALDI-TOF-MS; NMR;
D O I
10.1046/j.1432-1033.2002.03062.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bacteroides vulgatus has been shown to be involved in the aggravation of colitis. Previously, we separated two potent virulence factors, capsular polysaccharide (CPS) and lipopolysaccharide (LPS), from a clinical isolate of B. vulgatus and characterized the structure of CPS. In this study, we elucidated the structures of O-antigen polysaccharide (OPS) and lipid A in the LPS. LPS was subjected to weak acid hydrolysis to produce the lipid A fraction and polysaccharide fraction. Lipid A was isolated by preparative TLC, and its structure determined by MS and NMR to be similar to that of Bacteroides fragilis except for the number of fatty acids. The polysaccharide fraction was subjected to gel-filtration chromatography to give an OPS-rich fraction. The structure of OPS was determined by chemical analysis and NMR spectroscopy to be a polysaccharide composed of the following repeating unit: [-->4)alpha-L-Rhap(1-->3)beta-D-Manp(1-->].
引用
收藏
页码:3715 / 3721
页数:7
相关论文
共 29 条
[1]   COLORIMETRIC ANALYSIS OF SUGARS [J].
ASHWELL, G .
METHODS IN ENZYMOLOGY, 1957, 3 :73-105
[2]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[3]   STRUCTURAL ELUCIDATION OF 2 CAPSULAR POLYSACCHARIDES FROM ONE STRAIN OF BACTEROIDES-FRAGILIS USING HIGH-RESOLUTION NMR-SPECTROSCOPY [J].
BAUMANN, H ;
TZIANABOS, AO ;
BRISSON, JR ;
KASPER, DL ;
JENNINGS, HJ .
BIOCHEMISTRY, 1992, 31 (16) :4081-4089
[4]   C-13 NUCLEAR MAGNETIC-RESONANCE DATA FOR OLIGOSACCHARIDES [J].
BOCK, K ;
PEDERSEN, C ;
PEDERSEN, H .
ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1984, 42 :193-225
[5]   BACTEROIDES-VULGATUS OUTER-MEMBRANE ANTIGENS ASSOCIATED WITH CARRAGEENAN-INDUCED COLITIS IN GUINEA-PIGS [J].
BREELING, JL ;
ONDERDONK, AB ;
CISNEROS, RL ;
KASPER, DL .
INFECTION AND IMMUNITY, 1988, 56 (07) :1754-1759
[6]   A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].
CIUCANU, I ;
KEREK, F .
CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217
[7]   A REAPPRAISAL OF THE BIOLOGICAL-ACTIVITY OF BACTEROIDES LPS [J].
DELAHOOKE, DM ;
BARCLAY, GR ;
POXTON, IR .
JOURNAL OF MEDICAL MICROBIOLOGY, 1995, 42 (02) :102-112
[8]   EXPERIMENTAL-MODELS OF INFLAMMATORY BOWEL-DISEASE [J].
ELSON, CO ;
SARTOR, RB ;
TENNYSON, GS ;
RIDDELL, RH .
GASTROENTEROLOGY, 1995, 109 (04) :1344-1367
[9]   PURIFICATION AND FRACTIONATION OF LIPOPOLYSACCHARIDE FROM GRAM-NEGATIVE BACTERIA BY HYDROPHOBIC INTERACTION CHROMATOGRAPHY [J].
FISCHER, W .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 194 (02) :655-661
[10]   Structural elucidation of a capsular polysaccharide from a clinical isolate of Bacteroides vulgatus from a patient with Crohn's disease [J].
Hashimoto, M ;
Kirikae, F ;
Dohi, T ;
Kusumoto, S ;
Suda, Y ;
Kirikae, T .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (11) :3139-3144