Age-related decrease in the inducibility of heat-shock protein 70 in human peripheral blood mononuclear cells

被引:73
作者
Njemini, R
Vanden Abeele, M
Demanet, C
Lambert, M
Vandebosch, S
Mets, T
机构
[1] Free Univ Brussels, Acad Hosp, Geriatr Unit, B-1090 Brussels, Belgium
[2] Free Univ Brussels, Acad Hosp, HLA & Immunol Lab, B-1090 Brussels, Belgium
关键词
peripheral mononuclear blood cells; heat shock; heat-shock protein 70; aging; proinflammatory cytokines;
D O I
10.1023/A:1016036724386
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have investigated the effect of age and of the presence of proinflammatory cytokines on Hsp 70 production in human peripheral blood mononuclear cells, using flow cytometry. Twenty-seven women and 23 men, all apparently healthy, participated in the study. At 37degreesC, the percentage of Hsp 70-producing monocytes and lymphocytes, as well as the level of Hsp 70 in monocytes, were negatively influenced by age. After exposure of the cells to 42degreesC, the increase of Hsp 70 production was more pronounced in monocytes than in lymphocytes; both the intensity of Hsp 70 production and the percentage of Hsp 70-producing cells were negatively influenced by the age of the subjects, as well for monocytes as for lymphocytes. There was a negative correlation between the intensity of Hsp 70 production by monocytes exposed to 42degreesC and the serum levels of tumor necrosis factor-alpha and interleukin-6. In conclusion, in human monocytes and lymphocytes, heat-induced Hsp 70 production is reduced with increasing age and is negatively influenced in monocytes by proinflammatory cytokines.
引用
收藏
页码:195 / 205
页数:11
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