Detrimental effects of high molecular-mass polyphenols on olive mill wastewater biotreatment

Research was undertaken to investigate whether low, medium or high molecular-mass polyphenolics were the most problematic compounds for olive mill wastewater (OMW) biotreatments. For this reason, three polyphenolic fractions were isolated by ultrafiltration of crude OMW and characterized by GC/MS and gel filtration analysis. When P. chrysosporium was cultivated in the presence of Fl polyphenolic fraction (F1<8 kDa), the decolorization/depolymerization was high. However, a very weak decolorization/depolymerization of the F3 fraction (F3 > 60 kDa) was observed. Lignin peroxidase (LiP) was detected as traces for the Fl fraction and undetected in the case of F3 one. Moreover, increasing the molecular-mass of aromatics led to decreases in levels of depolymerization and COD removal by P. chrysosporium cultures. The use of a lignin peroxidase induction medium has a positive effect on the high molecular-mass fraction decolorization and a high degree of decolorization paralleled the appearance of Lip activity in the extracellular fluid. By comparison to the synthetic medium used, LiP production was delayed in the presence of Fl fraction but strongly inhibited (80% loss) in the presence of F3 fraction. The Fl fraction was well degraded aerobically by P. putida DSM3932 (pWWO) and A, eutrophus DSM 4057 (pJP3) or an activated sludge consortium while F3 resisted degradation by the OMW-acclimated activated sludge. Moreover, continuous anaerobic biomethanization experiments conducted in parallel with the different fractions showed that at the same hydraulic retention time (26 days), the Fl fraction was bioconverted into methane at high yield while F3 fraction was poorly biodegraded. (C) 2000 Elsevier Science Ltd. All rights reserved.