Early expression of Ig mu chain from a transgene significantly reduces the duration of the pro-B stage but does not affect the small pre-B stage

被引:14
作者
Arakawa, H [1 ]
Takeda, S [1 ]
机构
[1] BASEL INST IMMUNOL, CH-4005 BASEL, SWITZERLAND
关键词
B cell development; gene rearrangement; Ig; kappa chain; mu chain; V-J recombination;
D O I
10.1093/intimm/8.8.1319
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
During B cell development, V-J rearrangements at the Ig heavy mu chain (IgH mu chain) locus occur in early cycling precursors (pro-B stage), Subsequently, rearrangements at the Ig light (IgL) chain locus occur in late resting precursors (small pre-B stage), To study the effects of mu chain expression on the rate of B cell development, purified hematopoietic stem cells (HSC) bearing a mu chain transgene or wild-type HSC were transferred into immunodeficient RAG-2(-/-) mice and B cell development was followed over time, In addition, cycling B cell precursors were pulse-labeled by the injection of BrdU into transgenic and wild-type mice, and the production of BrdU-labeled kappa(+) and lambda(+) B cells was followed over time, These experiments suggested that early expression of the mu chain from the transgene significantly shortened the duration of the pro-B stage and immediately drove the precursors to differentiate into small pre-B cells, By contrast, the presence of the transgene did not affect the small pre-B stage, where IgL rearrangements occur, Thus, kappa and lambda, rearrangements occurred only after the arrest of cell cycling as previously shown in wild-type mice, even when the mu chain is artificially expressed earlier in B cell development.
引用
收藏
页码:1319 / 1328
页数:10
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