Expansion and delivery of human fibroblasts on micronized acellular dermal matrix for skin regeneration

被引:92
作者
Zhang, Xiaojun [1 ,4 ,5 ]
Deng, Zhihong [3 ]
Wang, Hailun [5 ,6 ]
Yang, Zhenhua [2 ,5 ]
Guo, Weihua [1 ,5 ]
Li, Yuan [1 ,5 ]
Ma, Dandan [1 ,5 ]
Yu, Chunyan [6 ]
Zhang, Yongjie [1 ,5 ]
Jin, Yan [1 ,5 ]
机构
[1] Fourth Mil Med Univ, Sch Stomatol, Dept Oral Histol & Pathol, Xian 710032, Peoples R China
[2] Fourth Mil Med Univ, Sch Stomatol, Dept Orthodont, Xian 710032, Peoples R China
[3] Fourth Mil Med Univ, Xijing Hosp, Dept Otolaryngol, Xian 710032, Peoples R China
[4] Fourth Mil Med Univ, Dept Phys & Math, Xian 710032, Peoples R China
[5] Fourth Mil Med Univ, Res & Dev Ctr Tissue Engn, Xian 710032, Peoples R China
[6] Fourth Mil Med Univ, Tangdu Hosp, Dept Dermatol, Xian 710038, Peoples R China
基金
国家高技术研究发展计划(863计划);
关键词
Micronized acellular dermal matrix; Expansion; Transplantation; Fibroblasts; Engineered particulate dermal substitute; IN-VIVO; STEM-CELLS; EXTRACELLULAR-MATRIX; BIOCOMPATIBILITY; SCAFFOLD; CULTURE; ADM; MICROCARRIER; DEGRADATION; GROWTH;
D O I
10.1016/j.biomaterials.2009.01.018
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
In order to obtain an abundant supply of autologous dermal fibroblasts for the manufacture of engineered autologous dermal substitutes, we fabricated the micronized acellular dermal matrix (MADM) microcarriers and expanded human fibroblasts on them. This novel approach eliminated the need for the repeated trypsinizations that may disrupt cell-extracellular matrix interactions and impair cell viability. This cell expansion protocol simultaneously formed an engineered particulate dermal substitute (EPDS) avoiding cell reseeded on the scaffolds process. We further tested its feasibility and effectiveness in athymic murine subcutaneous injection and full-thickness cutaneous wound model. Our results showed that MADM microcarriers retained the ultrastructure of the acellular dermal matrix, had good biocompatibility, and supported human fibroblast expansion either as a direct culture substrate or through Culturing cells in conditioned medium prepared from them. In the animal study, EPDS formed a thick layer of tissue below the subcutaneous muscle tissue at 3 weeks following EPDS injection into subcutaneous tissue. In full-thickness cutaneous wound, the degree of wound healing with EPDS implantation was better than that without EPDS although healing rates were not significantly different between Wounds implanted with or without EPDS. This demonstrates the potential utility of MADM not only as a cell Culture substrate to expand fibroblasts but also as a cell transplantation vehicle for skin regeneration, with several advantages over current expansion-transplantation protocols for skin regeneration. In addition, EPDS may be used for cosmetic or reconstructive Soft tissue augmentation in a minimally invasive fashion. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2666 / 2674
页数:9
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