Studies of keratins in tongue coating samples of hepatitis B patients by mass spectrometry

被引：11

作者：

Fang, Fang ^{[1
]}

Liu, Ping ^{[2
]}

Wang, Haoyang ^{[1
]}

Zhang, Li ^{[1
]}

Zhang, Jing ^{[1
]}

Gao, Yuping ^{[1
]}

Zeng, Liming ^{[1
]}

Guo, Yinlong ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai Mass Spectrometry Ctr, Shanghai 200032, Peoples R China

[2] Shanghai Univ Tradit Chinese Med, Inst Liver Dis, Shanghai 201203, Peoples R China

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2009年 / 23卷 / 11期

基金：

中国国家自然科学基金;

关键词：

TRADITIONAL CHINESE MEDICINE; QUANTITATIVE-ANALYSIS; IMAGE-ANALYSIS; ORAL MALODOR; LIVER-INJURY; PROTEINS; PERIODONTITIS; COMPLEXES; DIAGNOSIS; PROTEOME;

D O I：

10.1002/rcm.4060

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pooled tongue coating samples from 64 hepatitis B patients and 24 healthy adults were studied and a major band of differential proteins was found by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The differential proteins in this band were identified and proved to be keratins by liquid chromatography/tandem mass spectrometry (LC/MS/MS) and Western blot analysis. Furthermore, relative quantification of the identified keratins was performed via using stable isotopic labeling and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), showing the higher expression level of these keratins in tongue coating samples of hepatitis B patients than healthy adults. These results provided additional information to understand the medical diagnosis depending on the tongue coating. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：1703 / 1709

页数：7

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