Directed evolution: Creating biocatalysts for the future

被引：88

作者：

Arnold, FH

机构：

[1] Div. of Chem. and Chem. Eng. 210-41, California Institute of Technology, Pasadena

来源：

CHEMICAL ENGINEERING SCIENCE | 1996年 / 51卷 / 23期

关键词：

D O I：

10.1016/S0009-2509(96)00288-6

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

An effective approach to engineering new enzymes is to direct their evolution in vitro. By mimicking key processes of Darwinian evolution in the rest tube, the functions of enzymes can be explored free from the constraints of function within a living system. Efficient strategies for engineering new enzymes by multiple generations of random mutagenesis and recombination coupled with screening for improved variants have been developed. Our results with industrially important biocatalysts underscore the advantages of this 'evolutionary' approach to protein engineering. (From a talk presented at the first National Academy of Engineering ''Frontiers of Engineering'' Symposium, 21 September 1995.) Copyright (C) 1996 Elsevier Science Ltd

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页码：5091 / 5102

页数：12

相关论文

共 14 条

[1] DE-ESTERIFICATION OF CEPHALOSPORIN PARA-NITROBENZYL ESTERS BY MICROBIAL ENZYMES [J].

BRANNON, DR ;

MABE, JA ;

FUKUDA, DS .

JOURNAL OF ANTIBIOTICS, 1976, 29 (02) :121-124

[2] 3-DIMENSIONAL STRUCTURE OF D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE [J].

BUEHNER, M ;

FORD, GC ;

MORAS, D ;

OLSEN, KW ;

ROSSMANN, MG .

JOURNAL OF MOLECULAR BIOLOGY, 1974, 90 (01) :25-+

[3] ENZYME ENGINEERING FOR NONAQUEOUS SOLVENTS - RANDOM MUTAGENESIS TO ENHANCE ACTIVITY OF SUBTILISIN-E IN POLAR ORGANIC MEDIA [J].

CHEN, KQ ;

ARNOLD, FH .

BIO-TECHNOLOGY, 1991, 9 (11) :1073-1077

[4] TUNING THE ACTIVITY OF AN ENZYME FOR UNUSUAL ENVIRONMENTS - SEQUENTIAL RANDOM MUTAGENESIS OF SUBTILISIN-E FOR CATALYSIS IN DIMETHYLFORMAMIDE [J].

CHEN, KQ ;

ARNOLD, FH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5618-5622

[5] COMPLEX BETWEEN THE SUBTILISIN FROM A MESOPHILIC BACTERIUM AND THE LEECH INHIBITOR EGLIN-C [J].

DAUTER, Z ;

BETZEL, C ;

GENOV, N ;

PIPON, N ;

WILSON, KS .

ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE, 1991, 47 :707-&

[6] THE CRYSTAL-STRUCTURE OF HOLO-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM THE HYPERTHERMOPHILIC BACTERIUM THERMOTOGA-MAUITIMA AT 2.5-ANGSTROM RESOLUTION [J].

KORNDORFER, I ;

STEIPE, B ;

HUBER, R ;

TOMSCHY, A ;

JAENICKE, R .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 246 (04) :511-521

[7] Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents [J].

Moore, JC ;

Arnold, FH .

NATURE BIOTECHNOLOGY, 1996, 14 (04) :458-467

[8] PROTEIN MOTIFS .4. THE STRUCTURE AND EVOLUTION OF ALPHA/BETA BARREL PROTEINS [J].

REARDON, D ;

FARBER, GK .

FASEB JOURNAL, 1995, 9 (07) :497-503

[9] EVOLUTION IN ACTION [J].

SCANLAN, TS ;

REID, RC .

CHEMISTRY & BIOLOGY, 1995, 2 (02) :71-75

[10] STRUCTURE OF HOLO-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM BACILLUS-STEAROTHERMOPHILUS AT 1.8 A RESOLUTION [J].

SKARZYNSKI, T ;

MOODY, PCE ;

WONACOTT, AJ .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 193 (01) :171-187