Generation and enzymatic amplification of high-density functionalized DNA double strands

被引：81

作者：

Jäger, S ^{[1
]}

Famulok, M ^{[1
]}

机构：

[1] Univ Bonn, Kekule Inst Organ Chem & Biochem, Dipl Chem, D-53121 Bonn, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 25期

关键词：

DNA replication; nanostructures; polymerase chain reaction; polymerases;

D O I：

10.1002/anie.200453926

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Up to eight modifications could be incorporated into a DNA heteroduplex (see picture) by introducing different sets of 2′-deoxynucleotide derivatives through DNA polymerase mediated primer extension. All four natural nucleobases in each strand were substituted with different base-modified analogues. PCR conditions are described that allow the direct amplification of fully functionalized DNA double strands.

引用

页码：3337 / 3340

页数：4

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