Chromatographic characteristics of cholesterol-imprinted polymers prepared by covalent and non-covalent imprinting methods

被引:112
作者
Hwang, CC [1 ]
Lee, WC [1 ]
机构
[1] Natl Chung Cheng Univ, Dept Chem Engn, Chiayi 621, Taiwan
关键词
covalent imprinting; non-covalent imprinting; stationary phases; LC; molecular imprinting; molecularly imprinted polymers; cholesterol;
D O I
10.1016/S0021-9673(02)00559-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Cholesterol-imprinted polymers were prepared in bulk polymerization by the methods of covalent and non-covalent imprinting. The former involved the use of a template-containing monomer, cholesteryl (4-vinyl)phenyl carbonate, while the latter used the complexes of template and functional monomer, methacrylic acid or 4-vinylpyridine prior to polymerization. Columns packed with these molecularly imprinted polymers (MIPs) were all able to separate cholesterol from other steroids. For different combinations of cholesterol and beta-estradiol concentrations in a total of 1 g/l, the peak retention times for both compounds were nearly constant. The adsorption capacity for cholesterol onto the MIPs was found to significantly depend on the use of functional monomers, but the selectivity factors were only slightly different from each other at 2.9 to 3.2 since the separation was all based on the specific binding of cholesterol to recognition sites formed on the imprinted polymers. The capacity factors for cholesterol were determined to be 3.5, 4.0 and 3.1, respectively, for covalently imprinted, 4-vinylpyridine-based, and methacrylic acid-based non-covalently imprinted polymers. However, the covalently imprinted polymer was found to have a higher adsorption capacity for cholesterol and about fivefold higher chromatographic efficiency for cholesterol separation, in comparison with non-covalently imprinted polymers. The use of covalent imprinting significantly reduced the peak broadening and tailing. This advantage along with constant retention suggests that the covalently imprinted polymer has potential for quantitative analysis. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:69 / 78
页数:10
相关论文
共 36 条
[11]  
Kempe Maria, 1993, Journal of Molecular Recognition, V6, P25, DOI 10.1002/jmr.300060103
[12]   SUGAR BINDING POLYMERS SHOWING HIGH ANOMERIC AND EPIMERIC DISCRIMINATION OBTAINED BY NONCOVALENT MOLECULAR IMPRINTING [J].
MAYES, AG ;
ANDERSSON, LI ;
MOSBACH, K .
ANALYTICAL BIOCHEMISTRY, 1994, 222 (02) :483-488
[13]   MOLECULAR IMPRINTING [J].
MOSBACH, K .
TRENDS IN BIOCHEMICAL SCIENCES, 1994, 19 (01) :9-14
[14]   The emerging technique of molecular imprinting and its future impact on biotechnology [J].
Mosbach, K ;
Ramstrom, O .
BIO-TECHNOLOGY, 1996, 14 (02) :163-170
[15]   RECENT ADVANCES IN THE PREPARATION AND USE OF MOLECULARLY IMPRINTED POLYMERS FOR ENANTIOMERIC RESOLUTION OF AMINO-ACID DERIVATIVES [J].
OSHANNESSY, DJ ;
EKBERG, B ;
ANDERSSON, LI ;
MOSBACH, K .
JOURNAL OF CHROMATOGRAPHY, 1989, 470 (02) :391-399
[16]   Surface imprinting of cholesterol on submicrometer core-shell emulsion particles [J].
Pérez, N ;
Whitcombe, MJ ;
Vulfson, EN .
MACROMOLECULES, 2001, 34 (04) :830-836
[17]   RECOGNITION SITES INCORPORATING BOTH PYRIDINYL AND CARBOXY FUNCTIONALITIES PREPARED BY MOLECULAR IMPRINTING [J].
RAMSTROM, O ;
ANDERSSON, LI ;
MOSBACH, K .
JOURNAL OF ORGANIC CHEMISTRY, 1993, 58 (26) :7562-7564
[18]   Study of the thermodynamics and mass transfer kinetics of two enantiomers on a polymeric imprinted stationary phase [J].
Sajonz, P ;
Kele, M ;
Zhong, GM ;
Sellergren, B ;
Guiochon, G .
JOURNAL OF CHROMATOGRAPHY A, 1998, 810 (1-2) :1-17
[19]   Imprinted polymers for selective adsorption of cholesterol from gastrointestinal fluids [J].
Sellergren, B ;
Wieschemeyer, J ;
Boos, KS ;
Seidel, D .
CHEMISTRY OF MATERIALS, 1998, 10 (12) :4037-4046
[20]   DIRECT DRUG DETERMINATION BY SELECTIVE SAMPLE ENRICHMENT ON AN IMPRINTED POLYMER [J].
SELLERGREN, B .
ANALYTICAL CHEMISTRY, 1994, 66 (09) :1578-1582