Biosensor for detection of hypoxanthine based on xanthine oxidase immobilized on chemically modified carbon paste electrode

被引：66

作者：

Hu, SS ^{[1
]}

Xu, CL

Luo, JH

Luo, J

Cui, DF

机构：

[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Elect Informat Ctr, Wuhan 430072, Peoples R China

[3] Tongji Med Univ, Union Hosp, Wuhan 430022, Peoples R China

[4] Chinese Acad Sci, Inst Elect, State Key Lab Transducer Technol, Beijing 100080, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 412卷 / 1-2期

关键词：

biosensor; modified electrode; hypoxanthine; xanthine oxidase;

D O I：

10.1016/S0003-2670(00)00748-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel hypoxanthine (Hx) sensor is constructed using a sodium montmorillonite-methyl viologen carbon paste modified electrode. Xanthine oxidase (XO) is immobilized within a polyaniline film on the electrode surface by electropolymerization. The hypoxanthine detection is based on the oxygen consumed due to the enzymatic reaction involving xanthine oxidase, Electrocatalytic reduction of oxygen and the response of the sensor are evaluated using cyclic voltammetry. The response of the sensor is linear in the range of 1 mu M-0.4 mM hypoxanthine and the minimum detectable level is 0.8 mu M. The sensor can be applied to the determination of hypoxanthine in fish meat, and it is effective for eliminating interferences from coexisting substances in the samples. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：55 / 61

页数：7

共 18 条

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