Development of a plasma 17α-hydroxyprogesterone time resolved-fluorescence immunoassay involving a new biotinylated tracer

被引：16

作者：

Boudi, A

Giton, F

Galons, H

Eulry, B

Villette, JM

Soliman, H

Boudou, P

Fiet, J ^{[1
]}

机构：

[1] Fac Pharm, Chim Organ Lab, F-75005 Paris, France

[2] Hop St Louis, Lab Biol Hormonal, F-75475 Paris 10, France

来源：

STEROIDS | 2000年 / 65卷 / 02期

关键词：

time-resolved fluoroimmunoassay; biotin; 17; alpha-hydroxyprogesterone;

D O I：

10.1016/S0039-128X(99)00089-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A biotinylated 17 alpha-hydroxyprogesterone probe (3) was prepared from 17 alpha-hydroxyprogesterone-3-carboxymethyloxime and conjugate obtained by acylation of biotinylaminopropylammonium trifluroacetate. This new tracer was used in the development of a 17 alpha-hydroxyprogesterone time-resolved fluoroimmunoassay using streptavidin-europium. The new method was compared to a long-standing radioimmunoassay method and found to be more sensitive and economical. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

收藏

页码：103 / 108

页数：6

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