Huperzine A protects rat pheochromocytoma cells against hydrogen peroxide-induced injury

被引：98

作者：

Xiao, XQ ^{[1
]}

Yang, JW ^{[1
]}

Tang, XC ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China

来源：

NEUROSCIENCE LETTERS | 1999年 / 275卷 / 02期

关键词：

Huperzine A; acetylcholinesterase inhibitor; free radicals; hydrogen peroxide; glutathione peroxidase; catalase; PC12; cells; malondialdehyde;

D O I：

10.1016/S0304-3940(99)00695-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effects of Huperzine A (HupA), a novel acetylcholinesterase inhibitor, on hydrogen peroxide (H2O2) induced cell lesion, level of lipid peroxidation and antioxidant enzyme activities were investigated in rat pheochromocytoma line PC12. Following a 6-h exposure of the cells to H2O2 (200 mu M), a marked reduction in cell survival and activities of glutathione peroxidase and catalase, as well as increased production of malondialdehyde (MDA) were observed. Pretreatment of the cells with HupA (0.1-10.0 mu M) prior to H2O2 exposure significantly elevated the cell survival and antioxidant enzyme activities and decreased the level of MDA. Our results indicated that in addition to its anticholinesterase effects, HupA had protective effects against free radical-induced cell toxicity, which might be beneficial for the treatment of Alzheimer's disease. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：73 / 76

页数：4