Recognition of a human sperm surface protein involved in the progesterone-initiated acrosome reaction by antisera against an endomembrane progesterone binding protein from porcine liver

被引：37

作者：

Buddhikot, M

Falkenstein, E

Wehling, M

Meizel, S ^{[1
]}

机构：

[1] Univ Calif Davis, Sch Med, Dept Cell Biol & Human Anat, Davis, CA 95616 USA

[2] Heidelberg Univ, Fac Clin Med Mannheim, Inst Clin Pharmacol, D-6800 Mannheim, Germany

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1999年 / 158卷 / 1-2期

关键词：

sperm; progesterone; liver (P-4)-binding protein; antisera; acrosome reaction;

D O I：

10.1016/S0303-7207(99)00173-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Antisera against a porcine liver endomembrane progesterone (P-4)-binding protein inhibited the P-4-initiated acrosome reaction (AR) but not the ionomycin-initiated AR of human sperm. Indirect: immunofluorescence studies detected antigen in the sperm head that moved during capacitation from a posterior head region to a midhead region. Moreover, the antisera detected a 44.6 kDa protein in western blots of sperm digitonin extracts. These results suggest that a sperm protein with at least partial homology to the liver endomembrane P-4-binding protein, is a putative P-4-receptor on the sperm plasma membrane involved in the P-4-initiated AR. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：187 / 193

页数：7

共 33 条

[1] Cell surface binding sites for progesterone on human spermatozoa [J].