Aptamer biosensor for lable-free detection of human immunoglobulin E based on surface plasmon resonance

被引：61

作者：

Kim, Yun Hwa ^{[1
]}

Kim, Jun Pyo ^{[1
]}

Han, Se Jong ^{[1
]}

Sim, Sang Jun ^{[1
]}

机构：

[1] Sungkyunkwan Univ, Dept Chem Engn, Nanoopt & Biomol Engn Natl Lab, Suwon 440746, South Korea

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 139卷 / 02期

关键词：

Immunoglobulin E (IgE); Surface plasmon resonance (SPR); Aptamer; Self-assembled monolayer (SAM); ALLERGY;

D O I：

10.1016/j.snb.2009.03.013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Aptasensor, an aptamer-based sensor for non-labeled detection of human immunoglobulin E (IgE), was developed using Surface plasmon resonance (SPR). Various oligo(ethylene glycol) mixtures of different molar ratios of EG(6)-COOH and EG(3)-OH were used for the construction of self-assembled monolayers (SAM). Direct assays and modified sandwich assays over a 1.0nM anti-IgE, aptamer-immobilized, gold chip, consisting of a 1:3 molar ratio of EG(6)-COOH:EG(3)-OH SAM, were investigated for real-time IgE detection. The linear ranges for both were 1.0-1000 ng/mL and the lowest amounts of detectable analytes in the samples were 3.44 and 2.07 ng/mL for direct and modified sandwich assays, respectively. These results for IgE detection Suggest that the aptamer offers the possibility of a simple and realistic biosensor platform based oil a SAM-fabricated SPR system. Furthermore, as an optic-biosensor, an SPR aptasensor Could be used for the diagnosis of allergies. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：471 / 475

页数：5

共 21 条

[1]

Binh N., 2007, METHODS, V42, P150

[2] Effects of space flights on human allergic status (IgE-mediated sensitivity) [J].

Buravkova, L. B. ;

Rykova, M. P. ;

Gertsik, Y. G. ;

Antropova, E. N. .

ACTA ASTRONAUTICA, 2007, 60 (4-7) :254-258

[3] A strategy for sensitivity and specificity enhancements in prostate specific antigen-α1-antichymotrypsin detection based on surface plasmon resonance [J].

Cao, C ;

Kim, JP ;

Kim, BW ;

Chae, H ;

Yoon, HC ;

Yang, SS ;

Sim, SJ .

BIOSENSORS & BIOELECTRONICS, 2006, 21 (11) :2106-2113

[4]

EUN JC, 2006, ANAL CHIM ACTA, V564, P82

[5] Complement and Fc-receptors in regulation of the antibody response [J].

Heyman, B .

IMMUNOLOGY LETTERS, 1996, 54 (2-3) :195-199

[6]

HIJIRI H, 2008, SENSORS, V8, P1090

[7] Evidence for two independent distributions of serum immunoglobulin E in atopic families: Cognate and non-cognate IgE [J].

Jackola, DR ;

Blumenthal, MN ;

Rosenberg, A .

HUMAN IMMUNOLOGY, 2004, 65 (01) :20-30

[8]

KING CL, 1991, J IMMUNOL, V146, P1478

[9] An aptamer-based quartz crystal protein biosensor [J].

Liss, M ;

Petersen, B ;

Wolf, H ;

Prohaska, E .

ANALYTICAL CHEMISTRY, 2002, 74 (17) :4488-4495

[10] Label-free protein biosensor based on aptamer-modified carbon nanotube field-effect transistors [J].

Maehashi, Kenzo ;

Katsura, Taiji ;

Kerman, Kagan ;

Takamura, Yuzuru ;

Matsumoto, Kazuhiko ;

Tamiya, Eiichi .

ANALYTICAL CHEMISTRY, 2007, 79 (02) :782-787

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