Detection of hepatitis C core antigen in the antibody negative 'window' phase of hepatitis C infection

被引:64
作者
Peterson, J
Green, G
Iida, K
Caldwell, B
Kerrison, P
Bernich, S
Aoyagi, K
Lee, SR
机构
[1] Ortho Clin Diagnost, Res & Dev, Raritan, NJ 08869 USA
[2] Tonen Corp, Oi, Saitama, Japan
关键词
D O I
10.1046/j.1423-0410.2000.7820080.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background and Objectives: Despite improvements in assays for anti-HCV, there remains a significant delay before the appearance of antibodies following infection, during which, circulating viral RNA is present. We have evaluated a prototype assay for the serological detection of hepatitis C virus (HCV) core antigen with specimens derived from the early phase of HCV infection. Materials and Methods: Serial specimens from 24 individuals undergoing HCV seroconversion were tested for the presence of anti-HCV, HCV RNA and HCV core antigen. Results: HCV antigen was detected at the same time as HCV RNA in 83% (20/24) cases. The mean time to the first detection of HCV antigen was approximately 1 day later than HCV RNA. Overall, 87% of HCV-RNA-positive specimens contained detectable HCV core antigen. Conclusion: These results indicate that HCV core antigen can be identified by routine serological ELISA in specimens from the early antibody-negative phase of HCV infection. A test for HCV core antigen may be a useful test for identifying window phase blood donations from antibody negative donors infected with HCV. Copyright (C) 2000 S. Karger AG, Basel.
引用
收藏
页码:80 / 85
页数:6
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