Comparison of MS/MS methods for protein identification from 2D-PAGE

被引：5

作者：

Arrigoni, Giorgio

Fernandez, Celine

Holm, Cecilia

Scigelova, Michaela

James, Peter

机构：

[1] Lund Univ, Dept Prot Technol, S-22100 Lund, Sweden

[2] Univ Padua, Dept Biol Chem, I-35100 Padua, Italy

[3] Lund Univ, Dept Mol Endocrinol, S-22100 Lund, Sweden

[4] Thermo Electron, Hemel Hempstead, England

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 09期

关键词：

two-dimensional electrophoresis; MALDI; ESI; HPLC-MS; protein identification;

D O I：

10.1021/pr0601281

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have compared the use of a low resolution MALDI-Ion Trap MS/MS and a high-resolution ESI-TOF-MS/MS for the analysis of spots from 2D gels. The main criteria were speed and accuracy of protein identification. The results obtained using the MALDI-MS/MS system are comparable to those from the LC-MS/MS system in terms of accuracy, but less low-level proteins are identified while the time required for the analysis is dramatically reduced.

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页码：2294 / 2300

页数：7

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