Identification of RAPD markers linked to linolenic acid genes in rapeseed

被引:37
作者
Jourdren, C
Barret, P
Horvais, R
Delourme, R
Renard, M
机构
[1] INRA, Station d'Amélioration des Plantes, F-35650 Le Rheu
关键词
doubled haploid; gene mapping; genetic control; linolenic acid; mutant; plant breeding; RAPD; rape seed; Brassica napus;
D O I
10.1007/BF00027487
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The inheritance of the low linolenic acid content (derivated from mutant lines) in rapeseed was investigated. Molecular techniques of gene mapping through RAPD markers were applied on a microspore-derived progeny from a high x low linolenic acid Fl hybrid. 'Bulked segregant analysis' made it possible to test rapidly number of RAPD primers. Two linkage groups of 6 markers (72.7 cM and 75.6 cM) were determined. Each corresponded to a major QTL which explained 24% and 30.7% of the total phenotypic variation of the linolenic acid content. It was confirmed that two independant mutations were implied in the low linonenic acid content.
引用
收藏
页码:351 / 357
页数:7
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