Multiple nucleosome positioning with unique rotational phasing on multimers of the light-responsive elements of pea rbcS-3A and rbcS-3.6 genes: comparison between experimental and theoretical mapping

被引：2

作者：

Alessi, R

Cacchione, S

DeSantis, P

Fua, M

Savino, M

机构：

[1] UNIV ROMA LA SAPIENZA,DIPARTIMENTO GENET & BIOL MOL,IST PASTEUR,FDN CENCI BOLOGNETTI,I-00185 ROME,ITALY

[2] UNIV ROMA LA SAPIENZA,DIPARTIMENTO CHIM,I-00185 ROME,ITALY

来源：

BIOPHYSICAL CHEMISTRY | 1997年 / 67卷 / 1-3期

关键词：

nucleosome positioning; rbcS light-responsive elements; LRE multimers; theoretical methods for nucleosome positioning;

D O I：

10.1016/S0301-4622(97)00035-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleosome positioning along two DNA tracts, corresponding to tetramers of the light-responsive elements of pea rbcS-3A and rbcS-3.6 genes, were studied by experimental (exonuclease III mapping and band shift electrophoresis) as well as theoretical methods. Multiple nucleosome positioning with unique rotational phase was derived from both methods in satisfactorily good agreement, if nucleosome dyad axis positions are considered. Theoretical and experimental distributions of nucleosome frequencies appear different, probably on account of DNA sequence dependent digestion kinetics of exonuclease III. (C) 1997 Elsevier Science B.V.

引用

页码：151 / 158

页数：8

共 15 条

[1]

BOFFELLI D, 1991, BIOPHYS CHEM, V39, P179

[2] DIFFERENT SUPERSTRUCTURAL FEATURES OF THE COMPLEXES BETWEEN SPERMINE AND THE LIGHT RESPONSIVE ELEMENTS OF THE 2 PEA GENES RBCS-3A AND RBCS-3.6 - GEL-ELECTROPHORESIS AND CIRCULAR-DICHROISM STUDIES [J].