SUPERSTRUCTURAL FEATURES OF THE UPSTREAM REGULATORY REGIONS OF 2 PEA RBCS GENES AND NUCLEOSOMES POSITIONING - THEORETICAL PREDICTION AND EXPERIMENTAL EVALUATION

被引：8

作者：

CACCHIONE, S

CERONE, MA

DESANTIS, P

SAVINO, M

机构：

[1] UNIV ROMA 1,DIPARTIMENTO GENET & BIOL MOLEC,I-00185 ROME,ITALY

[2] UNIV ROMA 1,DIPARTIMENTO CHIM,ROME,ITALY

来源：

BIOPHYSICAL CHEMISTRY | 1995年 / 53卷 / 03期

关键词：

DNA SUPERSTRUCTURES; NUCLEOSOME POSITIONING; RBCS GENES; THEORETICAL PREDICTION;

D O I：

10.1016/0301-4622(94)00105-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleosome positioning on two 384 bp DNA fragments, obtained from the upstream regulatory region of two pea rbcS genes, relevant in photoregulated transcription, was predicted using our theoretical method, based on the evaluation of the sequence dependent DNA bending energy. The theoretical prediction was checked by experimental evaluation of nucleosome positions after in vitro reconstitution, by mapping Exonuclease III-resistant borders and by digesting monomeric sequences with various restriction enzymes. Both approaches satisfactorily confirmed the theoretical predictions, showing that the nucleotide sequence intrinsic bendability has a dominant role in nucleosome positioning.

引用

页码：267 / 281

页数：15

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