Nitric oxide production of rat Leydig and Sertoli cells is stimulated by round spermatid factor(s)

被引：17

作者：

Fujisawa, M ^{[1
]}

Tatsumi, N ^{[1
]}

Fujioka, H ^{[1
]}

Kanzaki, M ^{[1
]}

Okuda, Y ^{[1
]}

Arakawa, S ^{[1
]}

Kamidono, S ^{[1
]}

机构：

[1] Kobe Univ, Sch Med, Dept Urol, Chuo Ku, Kobe, Hyogo 6570017, Japan

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2000年 / 160卷 / 1-2期

关键词：

nitric oxide; iNOS; Leydig cell; Sertoli cell; round spermatid;

D O I：

10.1016/S0303-7207(99)00257-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In this study, we provide evidence of cell-to-cell interaction between rat germ cells and Leydig or Sertoli cells in relation to nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) messenger RNA (mRNA) expression. As a result of being cultured in a round spermatid-conditioned medium (RSd-CM), NO production in both Leydig and Sertoli cells increased in proportion to the length of the culture period, iNOS mRNA expression in both types of cells also increased in a dose-dependent manner as a result of being cultured with RSd-CM. This increase was detected as early as 3 h and was maintained up to 24 h. In contrast, neither NO production nor iNOS mRNA increased in either type of cell following culture in a pachytene spermatocyte-conditioned medium (PS-CM). Our findings suggest that RSd may control NO production of Leydig and Sertoli cells. This cell-to-cell interaction may be an important mechanism of regulation of testicular function. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：99 / 105

页数：7

共 50 条

[1]

ADAMS ML, 1994, J PHARMACOL EXP THER, V269, P230

[2]

ADAMS ML, 1992, LIFE SCI, V50, P35

[3] Characterization of nerve growth factor precursor protein expression in rat round spermatids and the trophic effects of nerve growth factor in the maintenance of Sertoli cell viability [J].

Chen, Y ;

Dicou, E ;

Djakiew, D .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1997, 127 (02) :129-136

[4] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[5] Spermatocytes and round spermatids of rat testis: The difference between in vivo and in vitro protein patterns [J].

Cossio, G ;

Sanchez, JC ;

Wettstein, R ;

Hochstrasser, DF .

ELECTROPHORESIS, 1997, 18 (3-4) :548-552

[6] NITRIC-OXIDE SYNTHASE (NOS-I) IN LEYDIG-CELLS OF THE HUMAN TESTIS [J].

DAVIDOFF, MS ;

MIDDENDORFF, R ;

MAYER, B ;

HOLSTEIN, AF .

ARCHIVES OF HISTOLOGY AND CYTOLOGY, 1995, 58 (01) :17-30

[7]

de Kretser DM, 1998, HUM REPROD, V13, P1

[8] A GERM-CELL FACTOR(S) MODULATES PREPROENKEPHALIN GENE-EXPRESSION IN RAT SERTOLI CELLS [J].

FUJISAWA, M ;

BARDIN, CW ;

MORRIS, PL .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1992, 84 (1-2) :79-88

[9]

GALIERI M, 1984, J ANDROL, V5, P409

[10] CYTOKINES, ENDOTOXIN, AND GLUCOCORTICOIDS REGULATE THE EXPRESSION OF INDUCIBLE NITRIC-OXIDE SYNTHASE IN HEPATOCYTES [J].

GELLER, DA ;

NUSSLER, AK ;

DISILVIO, M ;

LOWENSTEIN, CJ ;

SHAPIRO, RA ;

WANG, SC ;

SIMMONS, RL ;

BILLIAR, TR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (02) :522-526

← 1 2 3 4 5 →