Nitric oxide production of rat Leydig and Sertoli cells is stimulated by round spermatid factor(s)

被引：17

作者：

Fujisawa, M ^{[1
]}

Tatsumi, N ^{[1
]}

Fujioka, H ^{[1
]}

Kanzaki, M ^{[1
]}

Okuda, Y ^{[1
]}

Arakawa, S ^{[1
]}

Kamidono, S ^{[1
]}

机构：

[1] Kobe Univ, Sch Med, Dept Urol, Chuo Ku, Kobe, Hyogo 6570017, Japan

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2000年 / 160卷 / 1-2期

关键词：

nitric oxide; iNOS; Leydig cell; Sertoli cell; round spermatid;

D O I：

10.1016/S0303-7207(99)00257-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In this study, we provide evidence of cell-to-cell interaction between rat germ cells and Leydig or Sertoli cells in relation to nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) messenger RNA (mRNA) expression. As a result of being cultured in a round spermatid-conditioned medium (RSd-CM), NO production in both Leydig and Sertoli cells increased in proportion to the length of the culture period, iNOS mRNA expression in both types of cells also increased in a dose-dependent manner as a result of being cultured with RSd-CM. This increase was detected as early as 3 h and was maintained up to 24 h. In contrast, neither NO production nor iNOS mRNA increased in either type of cell following culture in a pachytene spermatocyte-conditioned medium (PS-CM). Our findings suggest that RSd may control NO production of Leydig and Sertoli cells. This cell-to-cell interaction may be an important mechanism of regulation of testicular function. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：99 / 105

页数：7

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