The Ph-3 gene from Solanum pimpinellifolium encodes CC-NBS-LRR protein conferring resistance to Phytophthora infestans

被引：107

作者：

Zhang, Chunzhi ^{[1
]}

Liu, Lei ^{[1
]}

Wang, Xiaoxuan ^{[1
]}

Vossen, Jack ^{[2
]}

Li, Guangcun ^{[3
]}

Li, Tao ^{[1
]}

Zheng, Zheng ^{[1
]}

Gao, Jianchang ^{[1
]}

Guo, Yanmei ^{[1
]}

Visser, Richard G. F. ^{[2
]}

Li, Junming ^{[1
]}

Bai, Yuling ^{[2
]}

Du, Yongchen ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

[2] Univ Wageningen & Res Ctr, Wageningen UR Plant Breeding, NL-6708 PB Wageningen, Netherlands

[3] Shandong Acad Agr Sci, Inst Vegetables & Flowers, Jinan 250100, Peoples R China

来源：

THEORETICAL AND APPLIED GENETICS | 2014年 / 127卷 / 06期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

LATE BLIGHT RESISTANCE; POTATO LATE BLIGHT; POWDERY MILDEW RESISTANCE; DISEASE RESISTANCE; R-GENE; CULTIVATED POTATO; AVIRULENCE GENES; CLONAL LINEAGE; TOMATO HYBRID; EVOLUTION;

D O I：

10.1007/s00122-014-2303-1

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Ph-3 is the first cloned tomato gene for resistance to late blight and encodes a CC-NBS-LRR protein. Late blight, caused by Phytophthora infestans, is one of the most destructive diseases in tomato. The resistance (R) gene Ph-3, derived from Solanum pimpinellifolium L3708, provides resistance to multiple P. infestans isolates and has been widely used in tomato breeding programmes. In our previous study, Ph-3 was mapped into a region harbouring R gene analogues (RGA) at the distal part of long arm of chromosome 9. To further narrow down the Ph-3 interval, more recombinants were identified using the flanking markers G2-4 and M8-2, which defined the Ph-3 gene to a 26 kb region according to the Heinz1706 reference genome. To clone the Ph-3 gene, a bacterial artificial chromosome (BAC) library was constructed using L3708 and one BAC clone B25E21 containing the Ph-3 region was identified. The sequence of the BAC clone B25E21 showed that only one RGA was present in the target region. A subsequent complementation analysis demonstrated that this RGA, encoding a CC-NBS-LRR protein, was able to complement the susceptible phenotype in cultivar Moneymaker. Thus this RGA was considered the Ph-3 gene. The predicted Ph-3 protein shares high amino acid identity with the chromosome-9-derived potato resistance proteins against P. infestans (Rpi proteins).

引用

页码：1353 / 1364

页数：12