The determination of huperzine A in European Lycopodiaceae species by HPLC-UV-MS

被引：24

作者：

Borloz, A. ^{[1
]}

Marston, A. ^{[1
]}

Hostettmann, K. ^{[1
]}

机构：

[1] Univ Geneva, Univ Lausanne, Lab Pharmacognosie & Phytochim, Sect Sci Pharmaceut, CH-1211 Geneva 4, Switzerland

来源：

PHYTOCHEMICAL ANALYSIS | 2006年 / 17卷 / 05期

关键词：

HPLC-UV-MS; TLC; huperzine A; Lycopodiaceae; Huperzia selago;

D O I：

10.1002/pca.922

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A rapid qualitative HPLC-UV-MS method was developed for the detection of huperzine A in Lycopodiaceac species. HPLC coupled with mass spectrometry experiments allowed the identification of the alkaloid and enabled targeted analysis of complex matrices such as herbal extracts. Huperzine A was detected by single ion monitoring of the pseudomolecular ion [M + H](+) at m/z 243.2. The alkaloid was also detected on TLC in a bioautographic enzyme assay with acetylcholinesterase to show the activity of the compound. Four Lycopodiaceae species collected in Switzerland were tested by these methods and Huperzia selago (L.) Bernh. ex Schrank et Martius was the only species found to contain huperzine A. Copyright (c) 2006 John Wiley & Sons, Ltd.

引用

页码：332 / 336

页数：5

共 12 条

[1]

Cummings Jeffrey L., 2000, Int J Neuropsychopharmacol, V3, P21, DOI 10.1017/S1461145700001917

[2]

HUMAN MEMORY AND CHOLINERGIC SYSTEM - RELATIONSHIP TO AGING [J].