Frequent occurrence of identical heavy and light chain Ig rearrangements

被引:57
作者
Seidl, KJ
MacKenzie, JD
Wang, DN
Kantor, AB
Kabat, EA
Herzenberg, LA
Herzenberg, LA
机构
[1] COLUMBIA UNIV, DEPT MICROBIOL, NEW YORK, NY 10032 USA
[2] AMCELL CORP, SUNNYVALE, CA 94089 USA
[3] STANFORD UNIV, SCH MED, BECKMAN CTR B007, DEPT GENET, STANFORD, CA 94305 USA
关键词
B-1; cells; CD5; clonal expansion; dominant idiotype; FACS; Ig sequences; phosphatidylcholine; single cell; V(H)11; V-H region sequences; V-L region sequences;
D O I
10.1093/intimm/9.5.689
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Single-cell PGR analyses of expressed Ig H and L chain sequences presented here show that certain rearrangements occur repeatedly and account for a major segment of the well-studied repertoire of B-1 cell autoantibodies that mediate the lysis of bromelain-treated mouse erythrocytes, i.e. antibodies reactive with phosphatidylcholine (PtC), We repeatedly isolated at least 10 different types of V-H region rearrangements, involving three distinct germline genes, among FAGS-sorted PtC-binding B-1 cells from three strains of mice (C57BL/6J, BALB/c and C.B-17). The predominant rearrangement, V(H)11-DSP-J(H)1 (V(H)11 type 1), has been previously found in anti-PtC hybridomas in several studies, We show that within each of six mice from two strains (C57BL/6J and BALB/c), unique instances of IgH/IgL pairing arose either from different B cell progenitors prior to IgH rearrangement or from pre-B cells which expanded after IgH rearrangement but prior to IgL rearrangement, Together with other recurrent rearrangements described here, our findings demonstrate that clonal expansion of mature B cells cannot account for all repeated rearrangements. As suggested by initial studies of dominant idiotype expression, these findings confirm that clonal expansion is only one of the mechanisms contributing to the establishment of recurrent rearrangements.
引用
收藏
页码:689 / 702
页数:14
相关论文
共 83 条
[51]   SITE-SPECIFIC RECOMBINATION IN THE IMMUNE-SYSTEM [J].
LIEBER, MR .
FASEB JOURNAL, 1991, 5 (14) :2934-2944
[52]  
LINKE B, 1995, LEUKEMIA, V9, P840
[53]   LIMITS ON HEAVY-CHAIN JUNCTIONAL DIVERSITY CONTRIBUTE TO THE RECURRENCE OF AN ANTIBODY VARIABLE REGION [J].
MANSER, T .
MOLECULAR IMMUNOLOGY, 1990, 27 (06) :503-511
[54]   ALL T15 ID-POSITIVE ANTIBODIES (BUT NOT THE MAJORITY OF VHT15+ ANTIBODIES) ARE PRODUCED BY PERITONEAL CD5+ LYMPHOCYTES-B [J].
MASMOUDI, H ;
MOTASANTOS, T ;
HUETZ, F ;
COUTINHO, A ;
CAZENAVE, PA .
INTERNATIONAL IMMUNOLOGY, 1990, 2 (06) :515-520
[55]   RESTRICTED IMMUNOGLOBULIN VARIABLE REGION GENE USAGE BY NORMAL LY-1 (CD5+) B-CELLS THAT RECOGNIZE PHOSPHATIDYL CHOLINE [J].
MERCOLINO, TJ ;
LOCKE, AL ;
AFSHARI, A ;
SASSER, D ;
TRAVIS, WW ;
ARNOLD, LW ;
HAUGHTON, G .
JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 169 (06) :1869-1877
[56]   PHOSPHATIDYL CHOLINE IS RECOGNIZED BY A SERIES OF LY-1+ MURINE B-CELL LYMPHOMAS SPECIFIC FOR ERYTHROCYTE-MEMBRANES [J].
MERCOLINO, TJ ;
ARNOLD, LW ;
HAUGHTON, G .
JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 163 (01) :155-165
[57]   NORMAL MOUSE PERITONEUM CONTAINS A LARGE POPULATION OF LY-1+ (CD5) B-CELLS THAT RECOGNIZE PHOSPHATIDYL CHOLINE - RELATIONSHIP TO CELLS THAT SECRETE HEMOLYTIC ANTIBODY SPECIFIC FOR AUTOLOGOUS ERYTHROCYTES [J].
MERCOLINO, TJ ;
ARNOLD, LW ;
HAWKINS, LA ;
HAUGHTON, G .
JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 168 (02) :687-698
[58]   DECREASED SURFACE IGM RECEPTOR-MEDIATED ACTIVATION OF PHOSPHOLIPASE C-GAMMA-2 IN B-1 LYMPHOCYTES [J].
MORRIS, DL ;
ROTHSTEIN, TL .
INTERNATIONAL IMMUNOLOGY, 1994, 6 (07) :1011-1016
[59]  
Osmond D G, 1990, Semin Immunol, V2, P173
[60]  
PARKS DR, 1986, HDB EXPT IMMUNOLOGY