Deciphering the renal code: Advances in conditional gene targeting

被引：11

作者：

Gawlik, A ^{[1
]}

Quaggin, SE

机构：

[1] Univ Toronto, Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[2] Univ Hosp Aachen, Dept Med 2, D-52070 Aachen, Germany

[3] Univ Toronto, St Michaels Hosp, Dept Med, Div Nephrol, Toronto, ON M5B 1W8, Canada

来源：

PHYSIOLOGY | 2004年 / 19卷

关键词：

D O I：

10.1152/physiol.00009.2004

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Several powerful new techniques can examine gene function in mammals. Recombinase systems and kidney-specific promoters enable gene knockout and overexpression. Genetic systems induced on administration or removal of antibiotics or hormones permit control of gene expression. Gene silencing using short interfering RNA expression systems should accelerate loss-of-function studies. Thorough characterization of animals that have undergone conditional gene targeting has already provided insights into renal development and diseases. Here we discuss the advantages and pitfalls of currently available gene-targeting systems.

引用

页码：245 / 252

页数：8

共 45 条

[1] Site-specific cassette exchange and germline transmission with mouse ES cells expressing φC31 integrase [J].

Belteki, G ;

Gertsenstein, M ;

Ow, DW ;

Nagy, A .

NATURE BIOTECHNOLOGY, 2003, 21 (03) :321-324

[2] Role for a bidentate ribonuclease in the initiation step of RNA interference [J].

Bernstein, E ;

Caudy, AA ;

Hammond, SM ;

Hannon, GJ .

NATURE, 2001, 409 (6818) :363-366

[3] A system for stable expression of short interfering RNAs in mammalian cells [J].

Brummelkamp, TR ;

Bernards, R ;

Agami, R .

SCIENCE, 2002, 296 (5567) :550-553

[4] Improved properties of FLP recombinase evolved by cycling mutagenesis [J].

Buchholz, F ;

Angrand, PO ;

Stewart, AF .

NATURE BIOTECHNOLOGY, 1998, 16 (07) :657-662

[5] Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele [J].

Carmeliet, P ;

Ferreira, V ;

Breier, G ;

Pollefeyt, S ;

Kieckens, L ;

Gertsenstein, M ;

Fahrig, M ;

Vandenhoeck, A ;

Harpal, K ;

Eberhardt, C ;

Declercq, C ;

Pawling, J ;

Moons, L ;

Collen, D ;

Risau, W ;

Nagy, A .

NATURE, 1996, 380 (6573) :435-439

[6] Germline transmission of RNAi in mice [J].

Carmell, MA ;

Zhang, LQ ;

Conklin, DS ;

Hannon, GJ ;

Rosenquist, TA .

NATURE STRUCTURAL BIOLOGY, 2003, 10 (02) :91-92

[7] Laser capture microscopy [J].

Curran, S ;

McKay, JA ;

McLeod, HL ;

Murray, GI .

JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY, 2000, 53 (02) :64-68

[8] Modification of gene activity in mouse embryos in utero by a tamoxifen-inducible form of Cre recombinase [J].

Danielian, PS ;

Muccino, D ;

Rowitch, DH ;

Michael, SK ;

McMahon, AP .

CURRENT BIOLOGY, 1998, 8 (24) :1323-1326

[9] Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice [J].

Dymecki, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) :6191-6196

[10] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

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