Deciphering the renal code: Advances in conditional gene targeting

被引:11
作者
Gawlik, A [1 ]
Quaggin, SE
机构
[1] Univ Toronto, Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
[2] Univ Hosp Aachen, Dept Med 2, D-52070 Aachen, Germany
[3] Univ Toronto, St Michaels Hosp, Dept Med, Div Nephrol, Toronto, ON M5B 1W8, Canada
关键词
D O I
10.1152/physiol.00009.2004
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Several powerful new techniques can examine gene function in mammals. Recombinase systems and kidney-specific promoters enable gene knockout and overexpression. Genetic systems induced on administration or removal of antibiotics or hormones permit control of gene expression. Gene silencing using short interfering RNA expression systems should accelerate loss-of-function studies. Thorough characterization of animals that have undergone conditional gene targeting has already provided insights into renal development and diseases. Here we discuss the advantages and pitfalls of currently available gene-targeting systems.
引用
收藏
页码:245 / 252
页数:8
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