Small interfering RNA and gene silencing in transgenic mice and rats

被引：185

作者：

Hasuwa, H

Kaseda, K

Einarsdottir, T

Okabe, M

机构：

[1] Osaka Univ, Genome Informat Res Ctr, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Microbial Dis Res Inst, Suita, Osaka 5650871, Japan

来源：

FEBS LETTERS | 2002年 / 532卷 / 1-2期

关键词：

RNA interference; small interfering RNA; gene silencing; RNA polymerase III promoter; transgenic animal;

D O I：

10.1016/S0014-5793(02)03680-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

After short duplexes of synthetic 21-23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector-based approach for siRNAs was demonstrated in mammalian cultured cell lines. However, the effect of RNA interference (RNAi) on various differentiated cells in live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein in every part of the mouse and rat body. These results suggest that transgenic RNAi could function as an alternative method of gene silencing by applying homologous recombination to embryonic stem (ES) cells, and should be successful even in species where ES cell lines remain unestablished. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：227 / 230

页数：4

共 20 条

[1] STRUCTURE AND TRANSCRIPTION OF A HUMAN-GENE FOR H1 RNA, THE RNA COMPONENT OF HUMAN RNASE-P [J].