Improving the efficiency of RNA interference in mammals

被引：198

作者：

Mittal, V ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, Canc Genome Res Ctr, Woodbury, NY 11797 USA

来源：

NATURE REVIEWS GENETICS | 2004年 / 5卷 / 05期

关键词：

D O I：

10.1038/nrg1323

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

RNA interference (RNAi) has been very successfully applied as a gene-silencing technology in both plants and invertebrates, but many practical obstacles need to be overcome before it becomes viable in mammalian systems. Greater specificity and efficiency of RNAi in mammals is being achieved by improving the design and selection of small interfering RNAs (siRNAs), by increasing the efficacy of their delivery to cells and organisms, and by engineering their conditional expression. Genome-wide functional RNAi screens, which are predominantly done in worms and flies, have now begun to revolutionize large-scale loss-of-function studies in mammals.

引用

页码：355 / 365

页数：11

共 127 条

[1] Lentiviral-mediated RNA interference [J].

Abbas-Terki, T ;

Blanco-Bose, W ;

Déglon, N ;

Pralong, W ;

Aebischer, P .

HUMAN GENE THERAPY, 2002, 13 (18) :2197-2201

[2]

AMOSNINO ME, 2003, CANCER RES, V63, P3539

[3] Adenoviral vectors expressing siRNAs for discovery and validation of gene function [J].

Arts, GJ ;

Langemeijer, E ;

Tissingh, R ;

Ma, LB ;

Pavliska, H ;

Dokic, K ;

Dooijes, R ;

Misic, E ;

Clasen, R ;

Michiels, F ;

van der Schueren, J ;

Lambrecht, M ;

Herman, S ;

Brys, R ;

Thys, K ;

Hoffmann, M ;

Tomme, P ;

van Es, H .

GENOME RESEARCH, 2003, 13 (10) :2325-2332

[4] Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes [J].

Ashrafi, K ;

Chang, FY ;

Watts, JL ;

Fraser, AG ;

Kamath, RS ;

Ahringer, J ;

Ruvkun, G .

NATURE, 2003, 421 (6920) :268-272

[5] Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening [J].

Aza-Blanc, P ;

Cooper, CL ;

Wagner, K ;

Batalov, S ;

Deveraux, QL ;

Cooke, MP .

MOLECULAR CELL, 2003, 12 (03) :627-637

[6] Retroviral delivery of small interfering RNA into primary cells [J].

Barton, GM ;

Medzhitov, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) :14943-14945

[7] A large-scale RNAi screen in human cells identifies new components of the p53 pathway [J].

Berns, K ;

Hijmans, EM ;

Mullenders, J ;

Brummelkamp, TR ;

Velds, A ;

Heimerikx, M ;

Kerkhoven, RM ;

Madiredjo, M ;

Nijkamp, W ;

Weigelt, B ;

Agami, R ;

Ge, W ;

Cavet, G ;

Linsley, PS ;

Beijersbergen, RL ;

Bernards, R .

NATURE, 2004, 428 (6981) :431-437

[8] Role for a bidentate ribonuclease in the initiation step of RNA interference [J].

Bernstein, E ;

Caudy, AA ;

Hammond, SM ;

Hannon, GJ .

NATURE, 2001, 409 (6818) :363-366

[9] The efficacy of small interfering RNAs targeted to the type 1 insulin-like growth factor receptor (IGF1R) is influenced by secondary structure in the IGF1R transcript [J].

Bohula, EA ;

Salisbury, AJ ;

Sohail, M ;

Playford, MP ;

Riedemann, J ;

Southern, EM ;

Macaulay, VM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (18) :15991-15997

[10] Genome-wide RNAi analysis of growth and viability in Drosophila cells [J].

Boutros, M ;

Kiger, AA ;

Armknecht, S ;

Kerr, K ;

Hild, M ;

Koch, B ;

Haas, SA ;

Paro, R ;

Perrimon, N .

SCIENCE, 2004, 303 (5659) :832-835

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