Excellent combination of counter-current chromatography and preparative high-performance liquid chromatography to separate galactolipids from pumpkin

被引：23

作者：

Du, Qizhen ^{[1
]}

Jiang, Zhiguo ^{[1
]}

Wang, Daijie ^{[1
]}

机构：

[1] Zhejiang Gongshang Univ, Inst Food & Biol Engn, Hangzhou 310035, Zhejiang, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 19期

关键词：

Galactolipids; Cucurbita moschata; Pumpkin; High-speed counter-current chromatography; Preparative HPLC; PROTEIN-BOUND POLYSACCHARIDE;

D O I：

10.1016/j.chroma.2008.11.066

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Galactolipids in the fruits of Cucurbita moschata (pumpkin) could not be completely separated by high-performance liquid chromatography (HPLC). Preparative HPLC was not available for preparing major galactolipid monomers in pumpkin. In the present paper, a combination of high-speed counter-current chromatography (HSCCC) and preparative HPLC was used for preparing the galactolipids. A fraction containing galactolipids (Fr60) from the purification of the n-butanol extract Of pumpkin by macro-porous resin column chromatography was first separated by HSCCC to result in three sub-fractions of each containing two galactolipid monomers. The three sub-fractions were further separated by preparative HPLC respectively to yield six galactolipid monomers with purity more than 96%. The method is a good one for preparing galactolipid monomers from plant materials for the studies of bioactivities. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：4176 / 4180

页数：5

共 12 条

[1]

Multiflorane triterpenoid esters from pumpkin. An unexpected extrafolic source of PABA [J].